CPLM-015154

Genbank Nucleotide ID

Mediator of RNA polymerase II transcription subunit 27

Protein Synonyms/Alias

Cofactor required for Sp1 transcriptional activation subunit 8; CRSP complex subunit 8; Mediator complex subunit 27; P37 TRAP/SMCC/PC2 subunit; Transcriptional coactivator CRSP34

Homo sapiens (Human)

Position	Peptide	Type	References
36	SRVFDCLKDGMRNKE	ubiquitination	[1]
50	ETLEGREKAFIAHFQ	ubiquitination	[1, 2, 3]
79	RLSNLVGKPSENHPL	ubiquitination	[4]
100	SLDPVQDKTPLYSQL	ubiquitination	[1]
112	SQLLQAYKWSNKLQY	ubiquitination	[2, 3, 4]
116	QAYKWSNKLQYHAGL	ubiquitination	[1, 2, 3]
134	LLNQQSLKRSANQMG	ubiquitination	[1, 4, 5, 6, 7]
145	NQMGVSAKRRPKAQP	ubiquitination	[1, 4]
215	FIDRTIVKGYNENVY	ubiquitination	[1, 4]
227	NVYTEDGKLDIWSKS	ubiquitination	[1, 4]
233	GKLDIWSKSNYQVFQ	ubiquitination	[2, 3]
275	TWLRSYIKLFQAPCQ	ubiquitination	[1, 2, 3, 7]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Activator; Alternative splicing; Complete proteome; Nucleus; Reference proteome; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IDA:HPA.
GO:0016592; C:mediator complex; IEA:Compara.
GO:0005730; C:nucleolus; IDA:HPA.
GO:0005667; C:transcription factor complex; IDA:MGI.
GO:0003713; F:transcription coactivator activity; IDA:UniProtKB.
GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IDA:MGI.
GO:0019827; P:stem cell maintenance; IEA:Compara.
GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.

IPR021627; Mediator_Med27.