CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013369
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 EH domain-containing protein 2 
Protein Synonyms/Alias
  
Gene Name
 Ehd2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
6**MFSWLKKGGARGQacetylation[1]
26RTVTSSLKELYRTKLacetylation[1]
32LKELYRTKLLPLEEHacetylation[1]
324EMPSVFGKENKKKQLacetylation[1]
334KKKQLIFKLPVIFAKacetylation[1]
370LMAHDFTKFHSLKPKacetylation[1]
375FTKFHSLKPKLLEALacetylation[1]
516ASHLIEAKLEGHGLPacetylation[1]
535RRLVPPSKRRQKGSAacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Required for translocation of FER1L5 to the plasma membrane. Binds ATP; does not bind GTP (By similarity). 
Sequence Annotation
 DOMAIN 449 537 EH.
 DOMAIN 481 516 EF-hand.
 NP_BIND 65 72 ATP (By similarity).
 BINDING 220 220 ATP (By similarity).
 BINDING 258 258 ATP (By similarity).
 MOD_RES 438 438 Phosphoserine (By similarity).
 MOD_RES 468 468 Phosphoserine (By similarity).  
Keyword
 ATP-binding; Calcium; Cell membrane; Complete proteome; Endosome; Hydrolase; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 543 AA 
Protein Sequence
MFSWLKKGGA RGQRSEAIRT VTSSLKELYR TKLLPLEEHY RFGSFHSPAL EDADFDGKPM 60
VLVAGQYSTG KTSFIQYLLE QEVPGSRVGP EPTTDCFVAV MHGETEGTVP GNALVVDPEK 120
PFRKLNPFGN TFLNRFMCAQ LPNQVLESIS IIDTPGILSG AKQRVSRGYD FPAVLRWFAE 180
RVDLIILLFD AHKLEISDEF SEAIGALRGH EDKIRVVLNK ADMVETQQLM RVYGALMWAL 240
GKVVGTPEVL RVYIGSFWSQ PLLVPDNRRL FELEEQDLFR DIQGLPRHAA LRKLNDLVKR 300
ARLVRVHAYI ISYLKKEMPS VFGKENKKKQ LIFKLPVIFA KIQLEHHISP GDFPDCQKMQ 360
ELLMAHDFTK FHSLKPKLLE ALDEMLTHDI AKLMPLLRQE ELESVEAGVQ GGAFEGTRMG 420
PFVERGPDEA IEDGEEGSED DAEWVVTKDK SKYDEIFYNL APADGKLSGS KAKTWMVGTK 480
LPNSVLGRIW KLSDVDRDGM LDDEEFALAS HLIEAKLEGH GLPTNLPRRL VPPSKRRQKG 540
SAE 543 
Gene Ontology
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0005886; C:plasma membrane; ISS:UniProtKB.
 GO:0055038; C:recycling endosome membrane; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005525; F:GTP binding; IEA:InterPro.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0030866; P:cortical actin cytoskeleton organization; IEA:Compara.
 GO:0032456; P:endocytic recycling; ISS:UniProtKB.
 GO:0006897; P:endocytosis; IEA:Compara.
 GO:0006184; P:GTP catabolic process; IEA:GOC.
 GO:2001137; P:positive regulation of endocytic recycling; ISS:UniProtKB.
 GO:1901741; P:positive regulation of myoblast fusion; ISS:UniProtKB.
 GO:0072659; P:protein localization to plasma membrane; ISS:UniProtKB. 
Interpro
 IPR001401; Dynamin_GTPase.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000261; EPS15_homology.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00350; Dynamin_N 
SMART
 SM00027; EH 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50031; EH 
PRINTS