CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021219
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Toll-interacting protein 
Protein Synonyms/Alias
  
Gene Name
 TOLLIP 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
63NITVVQAKLAKNYGMubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
66VVQAKLAKNYGMTRMubiquitination[2, 3, 4, 5, 6, 7, 8]
96PTAHNGAKNPRWNKVubiquitination[2, 3, 6, 7, 8]
146PESLRQGKVEDKWYSubiquitination[3, 6, 7]
150RQGKVEDKWYSLSGRubiquitination[2, 3, 5, 6, 8]
235RCSEEDLKAIQDMFPubiquitination[7]
Reference
 [1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. 
Sequence Annotation
 DOMAIN 40 135 C2.
 DOMAIN 229 272 CUE.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Immunity; Inflammatory response; Innate immunity; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 274 AA 
Protein Sequence
MATTVSTQRG PVYIGELPQD FLRITPTQQQ RQVQLDAQAA QQLQYGGAVG TVGRLNITVV 60
QAKLAKNYGM TRMDPYCRLR LGYAVYETPT AHNGAKNPRW NKVIHCTVPP GVDSFYLEIF 120
DERAFSMDDR IAWTHITIPE SLRQGKVEDK WYSLSGRQGD DKEGMINLVM SYALLPAAMV 180
MPPQPVVLMP TVYQQGVGYV PITGMPAVCS PGMVPVALPP AAVNAQPRCS EEDLKAIQDM 240
FPNMDQEVIR SVLEAQRGNK DAAINSLLQM GEEP 274 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0045323; C:interleukin-1 receptor complex; NAS:UniProtKB.
 GO:0045092; C:interleukin-18 receptor complex; NAS:UniProtKB.
 GO:0016604; C:nuclear body; IEA:Compara.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0004871; F:signal transducer activity; NAS:UniProtKB.
 GO:0007267; P:cell-cell signaling; TAS:ProtInc.
 GO:0006954; P:inflammatory response; NAS:UniProtKB.
 GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
 GO:0035556; P:intracellular signal transduction; NAS:UniProtKB.
 GO:0045321; P:leukocyte activation; NAS:UniProtKB.
 GO:0016310; P:phosphorylation; IDA:UniProtKB.
 GO:0033235; P:positive regulation of protein sumoylation; IEA:Compara. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR003892; CUE.
 IPR009060; UBA-like. 
Pfam
 PF00168; C2
 PF02845; CUE 
SMART
 SM00239; C2
 SM00546; CUE 
PROSITE
 PS50004; C2
 PS51140; CUE 
PRINTS