CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010052
UniProt Accession
Genbank Protein ID
 U00089 
Genbank Nucleotide ID
Protein Name
 Probable cytosol aminopeptidase 
Protein Synonyms/Alias
 Leucine aminopeptidase; LAP; Leucyl aminopeptidase 
Gene Name
 pepA 
Gene Synonyms/Alias
 MPN_572; MP270 
Created Date
 July 27, 2013 
Organism
 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
NCBI Taxa ID
 272634 
Lysine Modification
Position
Peptide
Type
References
5***MKLNKLFDSKTVacetylation[1]
10LNKLFDSKTVVFKKSacetylation[1]
15DSKTVVFKKSDKYGKacetylation[1]
19VVFKKSDKYGKSDCCacetylation[1]
22KKSDKYGKSDCCKTKacetylation[1]
29KSDCCKTKCVEGQIEacetylation[1]
57RALVTFLKTQKNQLNacetylation[1]
82KAENLCYKTALKVVVacetylation[1]
108TMKTEPQKNVEVAVKacetylation[1]
125SEHTSLIKEYEVVGNacetylation[1]
155LYPEEFVKRFEKAATacetylation[1]
159EFVKRFEKAATGLGVacetylation[1]
172GVKITVLKQADLIKKacetylation[1]
315DAISYAAKDLKATQIacetylation[1]
376PMHPDYLKPLESKLAacetylation[1]
Reference
 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819] 
Functional Description
 Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity). 
Sequence Annotation
 ACT_SITE 229 229 Potential.
 ACT_SITE 303 303 Potential.
 METAL 217 217 Manganese 2 (By similarity).
 METAL 222 222 Manganese 1 (By similarity).
 METAL 222 222 Manganese 2 (By similarity).
 METAL 240 240 Manganese 2 (By similarity).
 METAL 299 299 Manganese 1 (By similarity).
 METAL 301 301 Manganese 1 (By similarity).
 METAL 301 301 Manganese 2 (By similarity).  
Keyword
 Aminopeptidase; Complete proteome; Cytoplasm; Hydrolase; Manganese; Metal-binding; Protease; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 445 AA 
Protein Sequence
MKLNKLFDSK TVVFKKSDKY GKSDCCKTKC VEGQIEFGVK IPTDFPAFNR ALVTFLKTQK 60
NQLNVDLDSF VELYKAENLC YKTALKVVVA SITFCETTPF TMKTEPQKNV EVAVKCDSEH 120
TSLIKEYEVV GNYVNMARQL QDTPSDQLYP EEFVKRFEKA ATGLGVKITV LKQADLIKKK 180
MGLLLGVNKG SEREARLLVI SYNNNKKSSE TLALVGKGIT YDSGGMNIKT GDYMRGMKYD 240
MSGAAIVCST VLALAKNKVK TNVVAVAALT ENLPGPHAQR PDDIQTAYNG KTVEIDNTDA 300
EGRLVLADAI SYAAKDLKAT QIIDVATLTG LMSYILSTTY TGIFSTCDMA WDAFKKAACC 360
AGEPVWRLPM HPDYLKPLES KLADLQNSTS VKGAGSSRAA CFLAEFREGV PLIHCDIAST 420
ASIQDLGQGV LVRTLYERAA QQAKE 445 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004177; F:aminopeptidase activity; IEA:HAMAP.
 GO:0030145; F:manganese ion binding; IEA:HAMAP.
 GO:0008235; F:metalloexopeptidase activity; IEA:HAMAP.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 
Interpro
 IPR011356; Leucine_aapep/pepB.
 IPR000819; Peptidase_M17_C.
 IPR023042; Peptidase_M17_leu_NH2_pept. 
Pfam
 PF00883; Peptidase_M17 
SMART
  
PROSITE
 PS00631; CYTOSOL_AP 
PRINTS
 PR00481; LAMNOPPTDASE.