CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020318
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TBC1 domain family member 10A 
Protein Synonyms/Alias
 EBP50-PDX interactor of 64 kDa; EPI64 protein; Rab27A-GAP-alpha 
Gene Name
 TBC1D10A 
Gene Synonyms/Alias
 EPI64; TBC1D10 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
106NWDKWMAKKHKKIRLubiquitination[1]
107WDKWMAKKHKKIRLRubiquitination[1]
144KLQQNPGKFDELDMSubiquitination[1, 2]
265KVSPVAHKHLSRQKIubiquitination[1]
329LGSPEKVKACQGQYEubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Acts as GTPase-activating protein for RAB27A, but not for RAB2A, RAB3A, nor RAB4A. 
Sequence Annotation
 DOMAIN 111 299 Rab-GAP TBC.
 REGION 505 508 Binding to the PDZ domain of EBP50.
 MOD_RES 40 40 Phosphoserine.
 MOD_RES 407 407 Phosphoserine.  
Keyword
 Alternative splicing; Cell projection; Complete proteome; Direct protein sequencing; GTPase activation; Guanine-nucleotide releasing factor; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 508 AA 
Protein Sequence
MAKSNGENGP RAPAAGESLS GTRESLAQGP DAATTDELSS LGSDSEANGF AERRIDKFGF 60
IVGSQGAEGA PCPLLHRLEE VPLEVLRQRE SKWLDMLNNW DKWMAKKHKK IRLRCQKGIP 120
PSLRGRAWQY LSGGKVKLQQ NPGKFDELDM SPGDPKWLDV IERDLHRQFP FHEMFVSRGG 180
HGQQDLFRVL KAYTLYRPEE GYCQAQAPIA AVLLMHMPAE QAFWCLVQIC EKYLPGYYSE 240
KLEAIQLDGE ILFSLLQKVS PVAHKHLSRQ KIDPLLYMTE WFMCAFSRTL PWSSVLRVWD 300
MFFCEGVKII FRVGLVLLKH ALGSPEKVKA CQGQYETIER LRSLSPKIMQ EAFLVQEVVE 360
LPVTERQIER EHLIQLRRWQ ETRGELQCRS PPRLHGAKAI LDAEPGPRPA LQPSPSIRLP 420
LDAPLPGSKA KPKPPKQAQK EQRKQMKGRG QLEKPPAPNQ AMVVAAAGDA CPPQHVPPKD 480
SAPKDSAPQD LAPQVSAHHR SQESLTSQES EDTYL 515 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0005902; C:microvillus; TAS:UniProtKB.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
 GO:0005097; F:Rab GTPase activator activity; IEA:InterPro.
 GO:0097202; P:activation of cysteine-type endopeptidase activity; IDA:BHF-UCL.
 GO:0045862; P:positive regulation of proteolysis; IDA:BHF-UCL.
 GO:0032851; P:positive regulation of Rab GTPase activity; IEA:GOC. 
Interpro
 IPR000195; Rab-GTPase-TBC_dom. 
Pfam
 PF00566; RabGAP-TBC 
SMART
 SM00164; TBC 
PROSITE
 PS50086; TBC_RABGAP 
PRINTS