CPLM-023513

Genbank Nucleotide ID

STIP1 homology and U box-containing protein 1

Protein Synonyms/Alias

Carboxy terminus of Hsp70-interacting protein; E3 ubiquitin-protein ligase CHIP

Mus musculus (Mouse)

Position	Peptide	Type	References
42	NRLFVGRKYPEAAAC	acetylation	[1]

[1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]

Functional Description

E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF- BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation.

REPEAT 27 60 TPR 1.
REPEAT 61 94 TPR 2.
REPEAT 96 128 TPR 3.
DOMAIN 227 301 U-box.
MOD_RES 20 20 Phosphoserine.
MOD_RES 24 24 Phosphoserine.
MOD_RES 26 26 Phosphoserine.
MOD_RES 274 274 Phosphoserine (By similarity).
CROSSLNK 2 2 Glycyl lysine isopeptide (Lys-Gly)
CROSSLNK 23 23 Glycyl lysine isopeptide (Lys-Gly)
CROSSLNK 222 222 Glycyl lysine isopeptide (Lys-Gly)
CROSSLNK 256 256 Glycyl lysine isopeptide (Lys-Gly)

3D-structure; Complete proteome; Cytoplasm; DNA damage; DNA repair; Isopeptide bond; Ligase; Phosphoprotein; Reference proteome; Repeat; TPR repeat; Ubl conjugation; Ubl conjugation pathway.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; ISS:HGNC.
GO:0042405; C:nuclear inclusion body; IEA:Compara.
GO:0031371; C:ubiquitin conjugating enzyme complex; TAS:HGNC.
GO:0000151; C:ubiquitin ligase complex; ISS:UniProtKB.
GO:0030544; F:Hsp70 protein binding; ISS:HGNC.
GO:0051879; F:Hsp90 protein binding; ISS:BHF-UCL.
GO:0030674; F:protein binding, bridging; TAS:HGNC.
GO:0042803; F:protein homodimerization activity; IDA:HGNC.
GO:0046332; F:SMAD binding; ISS:HGNC.
GO:0030911; F:TPR domain binding; ISS:HGNC.
GO:0034450; F:ubiquitin-ubiquitin ligase activity; IDA:MGI.
GO:0071218; P:cellular response to misfolded protein; IMP:UniProtKB.
GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
GO:0006515; P:misfolded or incompletely synthesized protein catabolic process; IMP:UniProtKB.
GO:0090035; P:positive regulation of chaperone-mediated protein complex assembly; IEA:Compara.
GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; ISS:HGNC.
GO:0031398; P:positive regulation of protein ubiquitination; ISS:HGNC.
GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IMP:UniProtKB.
GO:0051865; P:protein autoubiquitination; ISS:UniProtKB.
GO:0006457; P:protein folding; TAS:HGNC.
GO:0070534; P:protein K63-linked ubiquitination; ISS:UniProtKB.
GO:0051604; P:protein maturation; TAS:HGNC.
GO:0031943; P:regulation of glucocorticoid metabolic process; ISS:HGNC.
GO:0030579; P:ubiquitin-dependent SMAD protein catabolic process; ISS:HGNC.

IPR001440; TPR-1.
IPR013026; TPR-contain_dom.
IPR011990; TPR-like_helical.
IPR019734; TPR_repeat.
IPR003613; Ubox_domain.
IPR013083; Znf_RING/FYVE/PHD.

PF00515; TPR_1
PF04564; U-box

SM00028; TPR
SM00504; Ubox

PS50005; TPR
PS50293; TPR_REGION