CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031888
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Echinoderm microtubule associated protein like 3, isoform CRA_e 
Protein Synonyms/Alias
 Echinoderm microtubule-associated protein-like 3 
Gene Name
 EML3 
Gene Synonyms/Alias
 hCG_20389 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
174PRQKLSRKAISSANLubiquitination[1]
194STESRGGKDPLSSPGubiquitination[1]
578ELLVGTTKNALLRGDubiquitination[1, 2, 3, 4, 5, 6, 7, 8, 9]
818AVADDFCKVHLFQYPubiquitination[9]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 897 AA 
Protein Sequence
MDGAAGPGDG PAREALQSLS QRLRVQEQEM ELVKAALAEA LRLLRLQVPP SSLQGSGTPA 60
PPGDSSLAAP PGLPPTCTPS LVSRGTQTET EVELKSSPGP PGLSNGPPAP QGASEEPSGT 120
QSEGGGSSSS GAGSPGPPGI LRPLQPPQRA DTPRRNSSSS SSPSERPRQK LSRKAISSAN 180
LLVRSGSTES RGGKDPLSSP GGPGSRRSNY NLEGISVKMF LRGRPITMYI PSGIRSLEEL 240
PSGPPPETLS LDWVYGYRGR DSRSNLFVLR SGEVVYFIAC VVVLYRPGGG PGGPGGGGQR 300
HYRGHTDCVR CLAVHPDGVR VASGQTAGVD KDGKPLQPVV HIWDSETLLK LQEIGLGAFE 360
RGVGALAFSA ADQGAFLCVV DDSNEHMLSV WDCSRGMKLA EIKSTNDSVL AVGFNPRDSS 420
CIVTSGKSHV HFWNWSGGVG VPGNGTLTRK QGVFGKYKKP KFIPCFVFLP DGDILTGDSE 480
GNILTWGRSP SDSKTPGRGG AKETYGIVAQ AHAHEGSIFA LCLRRDGTVL SGGGRDRRLV 540
QWGPGLVALQ EAEIPEHFGA VRAIAEGLGS ELLVGTTKNA LLRGDLAQGF SPVIQGHTDE 600
LWGLCTHPSQ NRFLTCGHDR QLCLWDGESH ALAWSIDLKE TGLCADFHPS GAVVAVGLNT 660
GRWLVLDTET REIVSDVIDG NEQLSVVRYS PDGLYLAIGS HDNVIYIYSV SSDGAKSSRF 720
GRCMGHSSFI THLDWSKDGN FIMSNSGDYE ILYWDVAGGC KQLKNRYESR DREWATYTCV 780
LGFHVYGVWP DGSDGTDINS LCRSHNERVV AVADDFCKVH LFQYPCARAK APSRMYGGHG 840
SHVTSVRFTH DDSHLVSLGG KDASIFQWRV LGAGGAGPAP ATPSRTPSLS PASSLDV 897 
Gene Ontology
  
Interpro
 IPR005108; HELP.
 IPR011047; Quinonprotein_ADH-like_supfam.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF03451; HELP
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS