UniProt Accession

 SMRCD_HUMAN; Q9H4L7; B7Z799; Q05D56; Q96SX1; Q9H017; Q9H860; Q9NPU9; Q9ULU7 

Genbank Protein ID

 AY008271; AB032948; AK023990; AK027490; AK301668; AC096746; BC017953; BC045534; AL359929; AL512768 

Genbank Nucleotide ID

 AAG16639.1; BAA86436.2; BAB14759.1; BAB55150.1; BAH13535.1; AAH17953.1; AAH45534.1; CAB95769.1; CAC21685.1 

Protein Name

 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 

Protein Synonyms/Alias

 ATP-dependent helicase 1; hHEL1 

Gene Name

 SMARCAD1 

Gene Synonyms/Alias

 KIAA1122 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
77	ETPDNERKASISYFK	ubiquitination	[1]
261	SIVLKLQKEFPNFDK	ubiquitination	[2]
410	LIPQCSQKKAQKITE	ubiquitination	[1, 3]
411	IPQCSQKKAQKITEL	ubiquitination	[1]
430	SWEALFTKMSKTNGL	ubiquitination	[1]
446	EDLIWHCKTLIQERD	ubiquitination	[1]
468	KCEDISNKLTKQVTM	ubiquitination	[1]
695	RMFSSKTKSADEQSI	ubiquitination	[1]
705	DEQSIYEKERIAHAK	ubiquitination	[1]
782	NVMMQLRKMANHPLL	ubiquitination	[1]
905	RYLRLDGKTQISERI	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs. Required for the restoration of heterochromatin organization after replication. Acts at replication sites to facilitate the maintenance of heterochromatin by directing H3 and H4 histones deacetylation, H3 'Lys-9' trimethylation (H3K9me3) and restoration of silencing. 

Sequence Annotation

 DOMAIN 157 199 CUE 1.
 DOMAIN 251 294 CUE 2.
 DOMAIN 509 677 Helicase ATP-binding.
 DOMAIN 858 1010 Helicase C-terminal.
 NP_BIND 521 529 ATP (By similarity).
 NP_BIND 897 904 ATP (By similarity).
 MOTIF 628 631 DEGH box.
 MOTIF 721 738 Nuclear localization signal (Potential).
 MOTIF 1005 1008 DEGD box.
 MOD_RES 54 54 Phosphothreonine.
 MOD_RES 57 57 Phosphoserine.
 MOD_RES 79 79 Phosphoserine.
 MOD_RES 124 124 Phosphoserine.
 MOD_RES 127 127 Phosphoserine.
 MOD_RES 132 132 Phosphoserine.
 MOD_RES 146 146 Phosphoserine.
 MOD_RES 152 152 Phosphoserine.
 MOD_RES 211 211 Phosphoserine.
 MOD_RES 212 212 Phosphoserine (By similarity).
 MOD_RES 213 213 Phosphoserine (By similarity).
 MOD_RES 214 214 Phosphoserine.
 MOD_RES 239 239 Phosphoserine.
 MOD_RES 242 242 Phosphoserine.
 MOD_RES 302 302 Phosphoserine.
 MOD_RES 408 408 Phosphoserine (By similarity).  

Keyword

 Alternative splicing; ATP-binding; Chromatin regulator; Chromosome; Complete proteome; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1026 AA 

Protein Sequence

Gene Ontology

 GO:0000792; C:heterochromatin; ISS:UniProtKB.
 GO:0016363; C:nuclear matrix; NAS:UniProtKB.
 GO:0043596; C:nuclear replication fork; IDA:UniProtKB.
 GO:0035861; C:site of double-strand break; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IDA:UniProtKB.
 GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
 GO:0043044; P:ATP-dependent chromatin remodeling; IMP:UniProtKB.
 GO:0051304; P:chromosome separation; IMP:UniProtKB.
 GO:0000729; P:DNA double-strand break processing; IMP:UniProtKB.
 GO:0070932; P:histone H3 deacetylation; IMP:UniProtKB.
 GO:0070933; P:histone H4 deacetylation; IMP:UniProtKB.
 GO:0009117; P:nucleotide metabolic process; NAS:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; NAS:UniProtKB.
 GO:0051260; P:protein homooligomerization; NAS:UniProtKB.
 GO:0000018; P:regulation of DNA recombination; IEP:UniProtKB. 

Interpro

 IPR003892; CUE.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000330; SNF2_N.
 IPR009060; UBA-like. 

Pfam

 PF00271; Helicase_C
 PF00176; SNF2_N 

SMART

 SM00487; DEXDc
 SM00490; HELICc 

PROSITE

 PS51140; CUE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 

PRINTS