CPLM-020501

Genbank Nucleotide ID

2,4-dienoyl-CoA reductase, mitochondrial

Protein Synonyms/Alias

2,4-dienoyl-CoA reductase [NADPH]; 4-enoyl-CoA reductase [NADPH]

Mus musculus (Mouse)

Position	Peptide	Type	References
36	TKTLYQSKDAPQSKF	acetylation	[1]
42	SKDAPQSKFFQPVLK	acetylation	[1, 2, 3, 4]
42	SKDAPQSKFFQPVLK	succinylation	[3]
49	KFFQPVLKPMLPPDA	acetylation	[1, 3, 4, 5, 6]
49	KFFQPVLKPMLPPDA	succinylation	[3]
73	GGGTGLGKAMTTFLS	acetylation	[3]
73	GGGTGLGKAMTTFLS	succinylation	[3]
97	SRNIDVLKATAEEIS	acetylation	[1, 2, 3, 4, 5, 7]
97	SRNIDVLKATAEEIS	succinylation	[3]
106	TAEEISSKTGNKVHA	acetylation	[1, 2, 3, 4, 8]
106	TAEEISSKTGNKVHA	succinylation	[3]
106	TAEEISSKTGNKVHA	ubiquitination	[9]
110	ISSKTGNKVHAIRCD	acetylation	[1, 4, 8]
133	NTVLELIKVAGHPDV	acetylation	[2]
185	EIGKQLIKAQKGAAF	acetylation	[2, 4]
244	IIQPGPIKTKGAFSR	acetylation	[1, 2, 3, 4]
244	IIQPGPIKTKGAFSR	succinylation	[3]
244	IIQPGPIKTKGAFSR	ubiquitination	[9]
246	QPGPIKTKGAFSRLD	acetylation	[1, 2, 4]
260	DPTGRFEKEMIDRIP	acetylation	[1, 2, 3, 4, 5, 7, 10, 11, 12]
260	DPTGRFEKEMIDRIP	succinylation	[3]
315	SGEFNSLKKVTKEEW	acetylation	[1, 2, 4]
315	SGEFNSLKKVTKEEW	ubiquitination	[9]
316	GEFNSLKKVTKEEWD	acetylation	[1, 2]
319	NSLKKVTKEEWDIIE	acetylation	[1, 2, 3, 4, 5]
319	NSLKKVTKEEWDIIE	succinylation	[3]

[1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
[2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
[5] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[7] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
[8] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
[9] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[10] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
[11] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
[12] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]

Functional Description

Auxiliary enzyme of beta-oxidation. It participates in the metabolism of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3- enoyl-CoA (By similarity).

NP_BIND 66 71 NADP (By similarity).
NP_BIND 240 243 NADP (By similarity).
ACT_SITE 199 199 Proton acceptor (Potential).
BINDING 91 91 NADP (By similarity).
BINDING 91 91 Substrate (By similarity).
BINDING 117 117 NADP (By similarity).
BINDING 119 119 Substrate (By similarity).
BINDING 149 149 Substrate (By similarity).
BINDING 214 214 NADP (By similarity).
BINDING 251 251 Substrate (By similarity).
MOD_RES 106 106 N6-acetyllysine.
MOD_RES 110 110 N6-acetyllysine.

Acetylation; Complete proteome; Fatty acid metabolism; Lipid metabolism; Mitochondrion; NADP; Oxidoreductase; Reference proteome; Transit peptide.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005739; C:mitochondrion; IDA:MGI.
GO:0005634; C:nucleus; IEA:Compara.
GO:0008670; F:2,4-dienoyl-CoA reductase (NADPH) activity; ISS:UniProtKB.
GO:0070402; F:NADPH binding; IEA:Compara.
GO:0006635; P:fatty acid beta-oxidation; ISS:UniProtKB.
GO:0051289; P:protein homotetramerization; IEA:Compara.

IPR002347; Glc/ribitol_DH.
IPR016040; NAD(P)-bd_dom.