CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006114
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ATP-dependent bile acid permease 
Protein Synonyms/Alias
  
Gene Name
 YBT1 
Gene Synonyms/Alias
 BAT1; YLL048C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
112NRHFSLEKLKSVKVNacetylation[1]
448KISTNKTKPSNEDPQubiquitination[2]
831DLTEIGEKGITLSGGubiquitination[2]
935FGEDELVKSSILSRAubiquitination[2]
951SSANLAAKSSTSLSNubiquitination[2]
979NSSHFEAKKLQKSLRubiquitination[2]
1655AKKAFVEKLNSKKD*ubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Vacuolar class C ABC transporter which regulates the translocation of phosphatidylcholine to the vacuole lumen, the release of lumenal calcium stores, and acts as a negative regulator of vacuole fusion. Exhibits ATP-dependent bile acid transport. 
Sequence Annotation
 DOMAIN 354 662 ABC transmembrane type-1 1.
 DOMAIN 694 935 ABC transporter 1.
 DOMAIN 1026 1345 ABC transmembrane type-1 2.
 DOMAIN 1381 1636 ABC transporter 2.
 NP_BIND 729 736 ATP 1 (Potential).
 NP_BIND 1415 1422 ATP 2 (Potential).
 MOD_RES 936 936 Phosphoserine.
 MOD_RES 940 940 Phosphoserine.
 MOD_RES 955 955 Phosphoserine.
 CARBOHYD 6 6 N-linked (GlcNAc...) (Potential).
 CARBOHYD 97 97 N-linked (GlcNAc...) (Potential).  
Keyword
 ATP-binding; Calcium; Calcium transport; Complete proteome; Glycoprotein; Ion transport; Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport; Vacuole. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1661 AA 
Protein Sequence
MHHVLNSTRP DHRFWFYDDV TQYGRTKYLN YYTPLVLLIF TVLFITYNIW KHYYYYDVLH 60
LKQKNPIDEL LYSSTDEDEQ SPLINNNTIT TNYVDNNCTK DALKNRHFSL EKLKSVKVNG 120
EPHGTPEIVR RGFIEKSRII LEFFLVLSQV IIHSFILLHY VNKNPEFTQQ GTITGLVEWC 180
ALFIIVSLRL ANVNQNFKFI NKYPGNLWSV SFINYLALFI SMILPFRSIF IHHINSPISR 240
KYYISQISIN LALFLLLFFA RIRNNFAIIY KTDSWITPSP EPVTSIAGFI CWAWLDSFVW 300
KAHKVSIKVK DIWGLMMQDY SFFVVKKFRY FVDHKVKRKR IFSLNLFFFF SNYLVLQCFW 360
AFLGSVLSFI PTVLLKRILE YVEDQSSAPS NLAWFYVTVM FVGRILVAIC QAQALFFGRR 420
VCIRMKSIII SEIYTKALRR KISTNKTKPS NEDPQEINDQ KSINGDEEST SSANLGAIIN 480
LMAIDAFKVS EICGYLHSFL EAFVMTVVAL ALLYRLLGFA AIVGVLIIVA MLPLNYKLAK 540
YIGDLQKKNL AVTDNRIQKL NEAFQAIRII KYFSWEENFE KDINTIRENE LSLLLMRSIV 600
WSISSFLWFV TPTIVTAASF AYYIYVQGEV LTTPVAFTAL SLFTLLRDPL DRLSDMLSFV 660
VQSKVSLDRV QDFLNENDTK KYDQLTIDPN GNRFAFENST ISWDKDNQDF KLKDLNIEFK 720
TGKLNVVIGP TGSGKTSLLM ALLGEMYLLN GKVVVPALEP RQELIVDANG TTNSIAYCSQ 780
AAWLLNDTVK NNILFNSPFN EARYKAVVEA CGLKRDFEIL KAGDLTEIGE KGITLSGGQK 840
QRVSLARALY SNARHVLLDD CLSAVDSHTA SWIYDNCITG PLMEDRTCIL VSHNIALTLR 900
NAELVVLLED GRVKDQGDPI DMLQKGLFGE DELVKSSILS RANSSANLAA KSSTSLSNLP 960
AVKEQQVSVN NNSSHFEAKK LQKSLRTEAE RTEDGKLIKE ETKEEGVVGL DVYKWYLKIF 1020
GGWKIVSFLA SLFLIAQLLY IGQSWWVRAW ASHNVIAKII PRAQRAIAFI SKKASHLIDW 1080
RGSSQISMAS AENQPSSGHS TMYYLVLYLI IGFAQALLGA GKTILNFVAG INASRKIFNM 1140
ILNKVLHSKI RFFDATPTGR IMNRFSKDIE AIDQELTPYI QGAFYSLIEC LSTVILITFI 1200
TPQFLSVAIV VSILYYFVGY FYMAGSRELK RFESISRSPI YQHFSETLVG VTTIRAFGDE 1260
GRFMQENLHK IDENNKPFFY LWVANRWLAF RIDMIGSLVI FGAGLFILFN INNLDSGMAG 1320
ISLTYAISFT EGALWLVRLY SEVEMNMNSV ERVKEYMEIE QEPYNEHKEI PPPQWPQDGK 1380
IEVNDLSLRY APNLPRVIKN VSFSVDAQSK IGIVGRTGAG KSTIITALFR FLEPETGHIK 1440
IDNIDISGVD LQRLRRSITI IPQDPTLFSG TIKTNLDPYD EFSDRQIFEA LKRVNLISEE 1500
QLQQGATRET SNEASSTNSE NVNKFLDLSS EISEGGSNLS QGQRQLMCLA RSLLRSPKII 1560
LLDEATASID YSSDAKIQET IRKEFQGSTI LTIAHRLRSV IDYDKILVMD AGEVKEYDHP 1620
YSLLLNKQSA FYSMCEHSGE LDILIELAKK AFVEKLNSKK D 1661 
Gene Ontology
 GO:0000324; C:fungal-type vacuole; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0015432; F:bile acid-exporting ATPase activity; IMP:SGD.
 GO:0006816; P:calcium ion transport; IEA:UniProtKB-KW. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR003439; ABC_transporter-like.
 IPR017871; ABC_transporter_CS.
 IPR017940; ABC_transporter_type1.
 IPR001140; ABC_transptr_TM_dom.
 IPR011527; ABC_transptrTM_dom_typ1.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00664; ABC_membrane
 PF00005; ABC_tran 
SMART
 SM00382; AAA 
PROSITE
 PS50929; ABC_TM1F
 PS00211; ABC_TRANSPORTER_1
 PS50893; ABC_TRANSPORTER_2 
PRINTS