CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019759
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kinesin-like protein KIF2C 
Protein Synonyms/Alias
 Kinesin-like protein 6; Mitotic centromere-associated kinesin; MCAK 
Gene Name
 KIF2C 
Gene Synonyms/Alias
 KNSL6 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
18LFPGLAIKIQRSNGLubiquitination[1]
52WAEGGATKGKEIDFDubiquitination[2]
54EGGATKGKEIDFDDVubiquitination[2]
90QENVTIQKQKRRSVNubiquitination[2, 3]
104NSKIPAPKESLRSRSubiquitination[2]
141PAAANSRKQFSVPPAubiquitination[1, 2]
196RRKSCLVKEVEKMKNubiquitination[2]
237WEFARMIKEFRATLEubiquitination[2]
275LNKQELAKKEIDVISacetylation[4]
276NKQELAKKEIDVISIacetylation[4]
276NKQELAKKEIDVISIubiquitination[1]
286DVISIPSKCLLLVHEubiquitination[2]
295LLLVHEPKLKVDLTKubiquitination[2]
297LVHEPKLKVDLTKYLubiquitination[2]
341QTIFEGGKATCFAYGubiquitination[2]
354YGQTGSGKTHTMGGDubiquitination[2]
365MGGDLSGKAQNASKGubiquitination[2, 3]
371GKAQNASKGIYAMASubiquitination[2]
385SRDVFLLKNQPCYRKubiquitination[2]
415KLFDLLNKKAKLRVLubiquitination[3]
416LFDLLNKKAKLRVLEubiquitination[2]
426LRVLEDGKQQVQVVGubiquitination[2, 3]
487AKGRMHGKFSLVDLAubiquitination[2]
518MEGAEINKSLLALKEubiquitination[2, 5, 6]
524NKSLLALKECIRALGubiquitination[1, 2]
534IRALGQNKAHTPFREubiquitination[2]
543HTPFRESKLTQVLRDubiquitination[2]
692SALAQQAKHFSALRDubiquitination[1, 2]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. 
Sequence Annotation
 DOMAIN 255 518 Kinesin-motor.
 NP_BIND 348 355 ATP.
 REGION 1 254 Globular (Potential).
 REGION 207 238 Negative regulator of microtubule-binding
 MOTIF 98 101 Microtubule tip localization signal.
 MOTIF 415 418 Nuclear localization signal (Potential).
 BINDING 264 264 ATP (By similarity).
 MOD_RES 22 22 Phosphoserine.
 MOD_RES 95 95 Phosphoserine; by AURKB.
 MOD_RES 109 109 Phosphoserine (By similarity).
 MOD_RES 111 111 Phosphoserine (By similarity).
 MOD_RES 115 115 Phosphoserine (By similarity).
 MOD_RES 166 166 Phosphoserine.
 MOD_RES 192 192 Phosphoserine (By similarity).
 MOD_RES 621 621 Phosphoserine.
 MOD_RES 633 633 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 725 AA 
Protein Sequence
MAMDSSLQAR LFPGLAIKIQ RSNGLIHSAN VRTVNLEKSC VSVEWAEGGA TKGKEIDFDD 60
VAAINPELLQ LLPLHPKDNL PLQENVTIQK QKRRSVNSKI PAPKESLRSR STRMSTVSEL 120
RITAQENDME VELPAAANSR KQFSVPPAPT RPSCPAVAEI PLRMVSEEME EQVHSIRGSS 180
SANPVNSVRR KSCLVKEVEK MKNKREEKKA QNSEMRMKRA QEYDSSFPNW EFARMIKEFR 240
ATLECHPLTM TDPIEEHRIC VCVRKRPLNK QELAKKEIDV ISIPSKCLLL VHEPKLKVDL 300
TKYLENQAFC FDFAFDETAS NEVVYRFTAR PLVQTIFEGG KATCFAYGQT GSGKTHTMGG 360
DLSGKAQNAS KGIYAMASRD VFLLKNQPCY RKLGLEVYVT FFEIYNGKLF DLLNKKAKLR 420
VLEDGKQQVQ VVGLQEHLVN SADDVIKMID MGSACRTSGQ TFANSNSSRS HACFQIILRA 480
KGRMHGKFSL VDLAGNERGA DTSSADRQTR MEGAEINKSL LALKECIRAL GQNKAHTPFR 540
ESKLTQVLRD SFIGENSRTC MIATISPGIS SCEYTLNTLR YADRVKELSP HSGPSGEQLI 600
QMETEEMEAC SNGALIPGNL SKEEEELSSQ MSSFNEAMTQ IRELEEKAME ELKEIIQQGP 660
DWLELSEMTE QPDYDLETFV NKAESALAQQ AKHFSALRDV IKALRLAMQL EEQASRQISS 720
KKRPQ 725 
Gene Ontology
 GO:0000775; C:chromosome, centromeric region; IDA:UniProtKB.
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005871; C:kinesin complex; TAS:ProtInc.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0019237; F:centromeric DNA binding; TAS:ProtInc.
 GO:0003777; F:microtubule motor activity; TAS:ProtInc.
 GO:0051010; F:microtubule plus-end binding; IDA:UniProtKB.
 GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; TAS:Reactome.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
 GO:0030951; P:establishment or maintenance of microtubule cytoskeleton polarity; IMP:HGNC.
 GO:0007019; P:microtubule depolymerization; IMP:UniProtKB.
 GO:0007018; P:microtubule-based movement; TAS:Reactome.
 GO:0007067; P:mitosis; TAS:ProtInc.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0051983; P:regulation of chromosome segregation; IMP:UniProtKB. 
Interpro
 IPR019821; Kinesin_motor_CS.
 IPR001752; Kinesin_motor_dom.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00225; Kinesin 
SMART
 SM00129; KISc 
PROSITE
 PS00411; KINESIN_MOTOR_DOMAIN1
 PS50067; KINESIN_MOTOR_DOMAIN2 
PRINTS
 PR00380; KINESINHEAVY.