UniProt Accession

 GGH_RAT; Q62867 

Genbank Protein ID

 U38379; BC087602 

Genbank Nucleotide ID

 AAC52506.1; AAH87602.1 

Protein Name

 Gamma-glutamyl hydrolase 

Protein Synonyms/Alias

 Conjugase; GH; Gamma-Glu-X carboxypeptidase 

Gene Name

 Ggh 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
265	SHAPNAVKTSFYLAK	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha- glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Exhibits either endo- or exopeptidase activity depending upon the tissue of origin. When secreted, it acts primarily as an endopeptidase. 

Sequence Annotation

 DOMAIN 25 317 Gamma-glutamyl hydrolase.
 ACT_SITE 133 133 Nucleophile (By similarity).
 ACT_SITE 243 243 Proton donor (By similarity).
 CARBOHYD 46 46 N-linked (GlcNAc...) (Potential).
 CARBOHYD 100 100 N-linked (GlcNAc...) (Potential).
 CARBOHYD 153 153 N-linked (GlcNAc...) (Potential).
 CARBOHYD 162 162 N-linked (GlcNAc...) (Potential).
 CARBOHYD 188 188 N-linked (GlcNAc...) (Potential).
 CARBOHYD 202 202 N-linked (GlcNAc...) (Potential).
 CARBOHYD 306 306 N-linked (GlcNAc...) (Potential).  

Keyword

 Complete proteome; Direct protein sequencing; Glycoprotein; Hydrolase; Lysosome; Reference proteome; Secreted; Signal. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 317 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:RGD.
 GO:0005615; C:extracellular space; IDA:RGD.
 GO:0005764; C:lysosome; TAS:RGD.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0034722; F:gamma-glutamyl-peptidase activity; ISS:UniProtKB.
 GO:0006541; P:glutamine metabolic process; IEA:InterPro.
 GO:0042493; P:response to drug; IDA:RGD.
 GO:0045471; P:response to ethanol; IDA:RGD.
 GO:0032868; P:response to insulin stimulus; IDA:RGD.
 GO:0010043; P:response to zinc ion; IDA:RGD. 

Interpro

 IPR015527; Pept_C26_g-glut_hydrolase.
 IPR011697; Peptidase_C26. 

Pfam

 PF07722; Peptidase_C26 

SMART

  

PROSITE

 PS51275; PEPTIDASE_C26_GGH 

PRINTS