UniProt Accession

 AIFM1_MOUSE; Q9Z0X1 

Genbank Protein ID

 AF100927; BC003292 

Genbank Nucleotide ID

 AAD16435.1; AAH03292.1 

Protein Name

 Apoptosis-inducing factor 1, mitochondrial 

Protein Synonyms/Alias

 Programmed cell death protein 8 

Gene Name

 Aifm1 

Gene Synonyms/Alias

 Aif; Pdcd8 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
108	LGLSPEEKQRRAIAS	ubiquitination	[1]
188	SDDPNVTKTLQFRQW	ubiquitination	[1]
243	DVRGNMVKLNDGSQI	acetylation	[2]
381	SGGRLLIKLKDGRKV	acetylation	[2]
445	AACFYDIKLGRRRVE	acetylation	[2]
517	ATAQDNPKSATEQSG	ubiquitination	[1]
592	PIARKIIKDGEQHED	acetylation	[2, 3]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647] 

Functional Description

 Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis,and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner (By similarity). 

Sequence Annotation

 NP_BIND 137 141 FAD.
 NP_BIND 163 164 FAD.
 NP_BIND 453 454 FAD.
 REGION 133 482 FAD-dependent oxidoreductase.
 MOTIF 445 450 Nuclear localization signal (Potential).
 BINDING 171 171 FAD.
 BINDING 176 176 FAD.
 BINDING 232 232 FAD; via amide nitrogen and carbonyl
 BINDING 284 284 FAD.
 BINDING 437 437 FAD.
 BINDING 482 482 FAD; via carbonyl oxygen.
 MOD_RES 267 267 Phosphoserine (By similarity).
 MOD_RES 592 592 N6-acetyllysine.
 CROSSLNK 254 254 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Acetylation; Apoptosis; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; FAD; Flavoprotein; Isopeptide bond; Membrane; Mitochondrion; Mitochondrion inner membrane; NAD; Nucleus; Oxidoreductase; Phosphoprotein; Reference proteome; Transit peptide; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 612 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
 GO:0005758; C:mitochondrial intermembrane space; ISS:UniProtKB.
 GO:0005741; C:mitochondrial outer membrane; TAS:MGI.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0004174; F:electron-transferring-flavoprotein dehydrogenase activity; TAS:MGI.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0006919; P:activation of cysteine-type endopeptidase activity involved in apoptotic process; ISS:UniProtKB.
 GO:0006309; P:apoptotic DNA fragmentation; IGI:MGI.
 GO:0008637; P:apoptotic mitochondrial changes; TAS:MGI.
 GO:0045454; P:cell redox homeostasis; IEA:InterPro.
 GO:0070059; P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; IMP:UniProtKB.
 GO:0032981; P:mitochondrial respiratory chain complex I assembly; IEA:Compara.
 GO:0051402; P:neuron apoptotic process; IGI:MGI.
 GO:0030182; P:neuron differentiation; IEA:Compara. 

Interpro

 IPR016156; FAD/NAD-linked_Rdtase_dimer.
 IPR013027; FAD_pyr_nucl-diS_OxRdtase.
 IPR004099; Pyr_nucl-diS_OxRdtase_dimer.
 IPR023753; Pyr_nucl-diS_OxRdtase_FAD/NAD.
 IPR001327; Pyr_OxRdtase_NAD-bd_dom. 

Pfam

 PF00070; Pyr_redox
 PF07992; Pyr_redox_2 

SMART

  

PROSITE

  

PRINTS

 PR00368; FADPNR.