CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006101
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 C-5 sterol desaturase 
Protein Synonyms/Alias
 Ergosterol Delta(5,6) desaturase; Sterol-C5-desaturase 
Gene Name
 ERG3 
Gene Synonyms/Alias
 PSO6; SYR1; YLR056W; L2150 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
56LQETLNSKNAVKECRubiquitination[1]
324DDSLFDPKLRDAKETubiquitination[2, 3, 4, 5, 6]
329DPKLRDAKETWDAQVubiquitination[1]
344KEVEHFIKEVEGDDNubiquitination[2, 4, 5]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [3] Systematic approach for validating the ubiquitinated proteome.
 Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
 Anal Chem. 2008 Jun 1;80(11):4161-9. [PMID: 18433149]
 [4] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [5] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [6] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Catalyzes the introduction of a C-5 double bond in the B ring of ergosterol. May contribute to the regulation of ergosterol biosynthesis. 
Sequence Annotation
 MOTIF 200 204 Histidine box-1.
 MOTIF 213 217 Histidine box-2.
 MOTIF 288 292 Histidine box-3.
 CROSSLNK 324 324 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 344 344 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Complete proteome; Endoplasmic reticulum; Iron; Isopeptide bond; Lipid biosynthesis; Lipid metabolism; Membrane; Oxidoreductase; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transmembrane; Transmembrane helix; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 365 AA 
Protein Sequence
MDLVLEVADH YVLDDLYAKV LPASLAANIP VKWQKLLGLN SGFSNSTILQ ETLNSKNAVK 60
ECRRFYGQVP FLFDMSTTSF ASLLPRSSIL REFLSLWVIV TIFGLLLYLF TASLSYVFVF 120
DKSIFNHPRY LKNQMAMEIK LAVSAIPWMS MLTVPWFVME LNGHSKLYMK IDYENHGVRK 180
LIIEYFTFIF FTDCGVYLAH RWLHWPRVYR ALHKPHHKWL VCTPFASHSF HPVDGFLQSI 240
SYHIYPLILP LHKVSYLILF TFVNFWTVMI HDGQYLSNNP AVNGTACHTV HHLYFNYNYG 300
QFTTLWDRLG GSYRRPDDSL FDPKLRDAKE TWDAQVKEVE HFIKEVEGDD NDRIYENDPN 360
TKKNN 365 
Gene Ontology
 GO:0005788; C:endoplasmic reticulum lumen; IDA:SGD.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0000248; F:C-5 sterol desaturase activity; IDA:SGD.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0006696; P:ergosterol biosynthetic process; IMP:SGD.
 GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro. 
Interpro
 IPR006694; Fatty_acid_hydroxylase. 
Pfam
 PF04116; FA_hydroxylase 
SMART
  
PROSITE
  
PRINTS