UniProt Accession

 BIOD1_ECOLI; P13000; Q2MBJ1 

Genbank Protein ID

 J04423; S68059; A11538; U00096; AP009048 

Genbank Nucleotide ID

 AAA23518.1; AAB29683.2; CAA00967.1; AAC73865.1; BAE76365.1 

Protein Name

 ATP-dependent dethiobiotin synthetase BioD 1 

Protein Synonyms/Alias

 DTB synthetase 1; DTBS 1; Dethiobiotin synthase 

Gene Name

 bioD1 

Gene Synonyms/Alias

 b0778; JW0761 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
28	CALLQAAKAAGYRTA	acetylation	[1]
46	PVASGSEKTPEGLRN	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes a mechanistically unusual reaction, the ATP- dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring. Only CTP can partially replace ATP while diaminobiotin is only 37% as effective as 7,8-diaminopelargonic acid. 

Sequence Annotation

 NP_BIND 13 18 ATP.
 NP_BIND 116 119 ATP.
 NP_BIND 176 177 ATP.
 NP_BIND 205 207 ATP.
 METAL 13 13 Magnesium 1.
 METAL 17 17 Magnesium 2.
 METAL 55 55 Magnesium 2.
 METAL 116 116 Magnesium 2.
 BINDING 42 42 Substrate.
 BINDING 55 55 ATP.
 BINDING 212 212 ATP.  

Keyword

 3D-structure; ATP-binding; Biotin biosynthesis; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 225 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0004141; F:dethiobiotin synthase activity; IDA:UniProtKB.
 GO:0000287; F:magnesium ion binding; IDA:UniProtKB.
 GO:0009102; P:biotin biosynthetic process; IDA:UniProtKB. 

Interpro

 IPR002586; CobQ/CobB/MinD/ParA_Nub-bd_dom.
 IPR004472; DTB_synth_BioD.
 IPR027417; P-loop_NTPase. 

Pfam

 PF01656; CbiA 

SMART

  

PROSITE

  

PRINTS