CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021581
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase pellino homolog 2 
Protein Synonyms/Alias
 Pellino-2 
Gene Name
 PELI2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
47KSRFALYKRPKANGVubiquitination[1]
69ISTPQASKAISCKGQubiquitination[1, 2]
74ASKAISCKGQHSISYubiquitination[1]
164AAGFDSSKNIFLGEKubiquitination[1]
171KNIFLGEKAAKWKNPubiquitination[1]
176GEKAAKWKNPDGHMDacetylation[3]
268LFHTPTQKHIEALRQubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates IL1B-induced IRAK1 'Lys-63'-linked polyubiquitination and possibly 'Lys-48'-linked ubiquitination. May be important for LPS- and IL1B-induced MAP3K7- dependent, but not MAP3K3-dependent, NF-kappa-B activation. Can activate the MAP (mitogen activated protein) kinase pathway leading to activation of ELK1. 
Sequence Annotation
 DOMAIN 15 202 FHA; atypical.  
Keyword
 3D-structure; Complete proteome; Ligase; Phosphoprotein; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 420 AA 
Protein Sequence
MFSPGQEEHC APNKEPVKYG ELVVLGYNGA LPNGDRGRRK SRFALYKRPK ANGVKPSTVH 60
VISTPQASKA ISCKGQHSIS YTLSRNQTVV VEYTHDKDTD MFQVGRSTES PIDFVVTDTI 120
SGSQNTDEAQ ITQSTISRFA CRIVCDRNEP YTARIFAAGF DSSKNIFLGE KAAKWKNPDG 180
HMDGLTTNGV LVMHPRGGFT EESQPGVWRE ISVCGDVYTL RETRSAQQRG KLVESETNVL 240
QDGSLIDLCG ATLLWRTADG LFHTPTQKHI EALRQEINAA RPQCPVGLNT LAFPSINRKE 300
VVEEKQPWAY LSCGHVHGYH NWGHRSDTEA NERECPMCRT VGPYVPLWLG CEAGFYVDAG 360
PPTHAFTPCG HVCSEKSAKY WSQIPLPHGT HAFHAACPFC ATQLVGEQNC IKLIFQGPID 420 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0002755; P:MyD88-dependent toll-like receptor signaling pathway; TAS:Reactome.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; TAS:Reactome.
 GO:0043410; P:positive regulation of MAPK cascade; IDA:MGI.
 GO:0001934; P:positive regulation of protein phosphorylation; IDA:MGI.
 GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
 GO:0008063; P:Toll signaling pathway; IEA:InterPro.
 GO:0034166; P:toll-like receptor 10 signaling pathway; TAS:Reactome.
 GO:0034134; P:toll-like receptor 2 signaling pathway; TAS:Reactome.
 GO:0034142; P:toll-like receptor 4 signaling pathway; TAS:Reactome.
 GO:0034146; P:toll-like receptor 5 signaling pathway; TAS:Reactome.
 GO:0034162; P:toll-like receptor 9 signaling pathway; TAS:Reactome.
 GO:0038123; P:toll-like receptor TLR1:TLR2 signaling pathway; TAS:Reactome.
 GO:0038124; P:toll-like receptor TLR6:TLR2 signaling pathway; TAS:Reactome. 
Interpro
 IPR006800; Pellino_fam. 
Pfam
 PF04710; Pellino 
SMART
  
PROSITE
 PS50006; FHA_DOMAIN 
PRINTS