| Tag | Content |
|---|
| CPLM ID | CPLM-007099 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Acetyl-coenzyme A synthetase |
Protein Synonyms/Alias | AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme |
Gene Name | acsA |
Gene Synonyms/Alias | BSU29680 |
Created Date | July 27, 2013 |
Organism | Bacillus subtilis (strain 168) |
NCBI Taxa ID | 224308 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 549 | LPKTRSGKIMRRVLK | acetylation | [1] |
|
Reference | [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis. Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG. Proteomics. 2013 May;13(10-11):1726-36. [ PMID: 23468065] |
Functional Description | Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA (By similarity). Has a role in growth and sporulation on acetate. |
Sequence Annotation | ACT_SITE 457 457 By similarity.
METAL 477 477 Magnesium; via carbonyl oxygen (By
METAL 479 479 Magnesium; via carbonyl oxygen (By
METAL 482 482 Magnesium; via carbonyl oxygen (By
BINDING 260 260 Coenzyme A (By similarity).
BINDING 333 333 Substrate; via amide nitrogen (By
BINDING 440 440 Substrate (By similarity).
BINDING 455 455 Substrate (By similarity).
BINDING 463 463 Coenzyme A (By similarity).
BINDING 466 466 Substrate (By similarity).
BINDING 524 524 Coenzyme A.
MOD_RES 549 549 N6-acetyllysine (By similarity). |
Keyword | Acetylation; ATP-binding; Complete proteome; Ligase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 572 AA |
Protein Sequence | MNLKALPAIE GDHNLKNYEE TYRHFDWAEA EKHFSWHETG KLNAAYEAID RHAESFRKNK 60
VALYYKDAKR DEKYTFKEMK EESNRAGNVL RRYGNVEKGD RVFIFMPRSP ELYFIMLGAI 120
KIGAIAGPLF EAFMEGAVKD RLENSEAKVV VTTPELLERI PVDKLPHLQH VFVVGGEAES 180
GTNIINYDEA AKQESTRLDI EWMDKKDGFL LHYTSGSTGT PKGVLHVHEA MIQQYQTGKW 240
VLDLKEEDIY WCTADPGWVT GTVYGIFAPW LNGATNVIVG GRFSPESWYG TIEQLGVNVW 300
YSAPTAFRML MGAGDEMAAK YDLTSLRHVL SVGEPLNPEV IRWGHKVFNK RIHDTWWMTE 360
TGSQLICNYP CMDIKPGSMG KPIPGVEAAI VDNQGNELPP YRMGNLAIKK GWPSMMHTIW 420
NNPEKYESYF MPGGWYVSGD SAYMDEEGYF WFQGRVDDVI MTSGERVGPF EVESKLVEHP 480
AIAEAGVIGK PDPVRGEIIK AFIALREGFE PSDKLKEEIR LFVKQGLAAH AAPREIEFKD 540
KLPKTRSGKI MRRVLKAWEL NLPAGDLSTM ED 572 |
Gene Ontology | |
Interpro | |
Pfam | |
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PROSITE | |
PRINTS | |