UniProt Accession

 DMA2_YEAST; P53924; B0KZS8; B0KZU6; B0KZW4; B0L009; D6W166; Q66GT0 

Genbank Protein ID

 EF125216; EF125217; EF125218; EF125219; EF125220; EF125221; EF125222; EF125223; EF125224; EF125225; EF125226; EF125228; Z69382; Z71392; BK005579; BK006947 

Genbank Nucleotide ID

 ABN58541.1; ABN58550.1; ABN58559.1; ABN58568.1; ABN58576.1; ABN58586.1; ABN58595.1; ABN58604.1; ABN58613.1; ABN58622.1; ABN58631.1; ABN58649.1; CAA93391.1; CAA95996.1; DAA05594.1; DAA10432.1 

Protein Name

 E3 ubiquitin-protein ligase DMA2 

Protein Synonyms/Alias

 Checkpoint forkhead associated with RING domains-containing protein 1; Defective in mitotic arrest protein 2 

Gene Name

 DMA2 

Gene Synonyms/Alias

 CHF2; YNL116W; N1925 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
288	FFEPIIRKAGPGSQL	ubiquitination	[1]
412	LKANSFNKEALQRLQ	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 E3 ubiquitin-protein ligase which functions in cell cycle retarding in conjonction with the UBC4 and UBC13/MMS2 complex, 2 E2 ubiquitin conjugationg enzymes. Involved in nutritional control of the cell cycle. Required for proper spindle positioning, likely regulating septin ring deposition at the bud neck. 

Sequence Annotation

 DOMAIN 295 358 FHA.
 ZN_FING 433 477 RING-type.
 MOD_RES 206 206 Phosphoserine.
 CROSSLNK 211 211 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 256 256 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 258 258 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 288 288 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 310 310 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 333 333 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 343 343 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 346 346 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 366 366 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 406 406 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 412 412 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 423 423 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Cell cycle; Cell division; Complete proteome; Cytoplasm; Isopeptide bond; Ligase; Metal-binding; Mitosis; Phosphoprotein; Reference proteome; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 522 AA 

Protein Sequence

Gene Ontology

 GO:0032177; C:cellular bud neck split septin rings; IMP:SGD.
 GO:0005934; C:cellular bud tip; IMP:SGD.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0032186; P:cellular bud neck septin ring organization; IGI:SGD.
 GO:0000132; P:establishment of mitotic spindle orientation; IGI:SGD.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0031578; P:mitotic spindle orientation checkpoint; IGI:SGD.
 GO:0051865; P:protein autoubiquitination; IDA:SGD.
 GO:0097271; P:protein localization to bud neck; IGI:SGD.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IGI:SGD.
 GO:0000921; P:septin ring assembly; IGI:SGD. 

Interpro

 IPR000253; FHA_dom.
 IPR008984; SMAD_FHA_domain.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD. 

Pfam

 PF00498; FHA 

SMART

 SM00240; FHA
 SM00184; RING 

PROSITE

 PS50006; FHA_DOMAIN
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 

PRINTS