CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002659
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Muscarinic acetylcholine receptor M1 
Protein Synonyms/Alias
  
Gene Name
 Chrm1 
Gene Synonyms/Alias
 Chrm-1 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
233QGSETPGKGGGSSSSubiquitination[1]
316SEAQAPTKQPPKSSPubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. 
Sequence Annotation
 BINDING 105 105 Antagonist.
 MOD_RES 428 428 Phosphothreonine (Potential).
 MOD_RES 451 451 Phosphoserine (Potential).
 MOD_RES 455 455 Phosphothreonine (Potential).
 MOD_RES 457 457 Phosphoserine (Potential).
 CARBOHYD 2 2 N-linked (GlcNAc...) (Probable).
 CARBOHYD 12 12 N-linked (GlcNAc...) (Probable).
 DISULFID 98 178 Probable.  
Keyword
 Cell junction; Cell membrane; Complete proteome; Direct protein sequencing; Disulfide bond; G-protein coupled receptor; Glycoprotein; Membrane; Phosphoprotein; Postsynaptic cell membrane; Receptor; Reference proteome; Synapse; Transducer; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 460 AA 
Protein Sequence
MNTSVPPAVS PNITVLAPGK GPWQVAFIGI TTGLLSLATV TGNLLVLISF KVNTELKTVN 60
NYFLLSLACA DLIIGTFSMN LYTTYLLMGH WALGTLACDL WLALDYVASN ASVMNLLLIS 120
FDRYFSVTRP LSYRAKRTPR RAALMIGLAW LVSFVLWAPA ILFWQYLVGE RTVLAGQCYI 180
QFLSQPIITF GTAMAAFYLP VTVMCTLYWR IYRETENRAR ELAALQGSET PGKGGGSSSS 240
SERSQPGAEG SPESPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV 300
VIKMPMVDSE AQAPTKQPPK SSPNTVKRPT KKGRDRGGKG QKPRGKEQLA KRKTFSLVKE 360
KKAARTLSAI LLAFILTWTP YNIMVLVSTF CKDCVPETLW ELGYWLCYVN STVNPMCYAL 420
CNKAFRDTFR LLLLCRWDKR RWRKIPKRPG SVHRTPSRQC 460 
Gene Ontology
 GO:0032279; C:asymmetric synapse; IDA:RGD.
 GO:0043679; C:axon terminus; IDA:RGD.
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0005887; C:integral to plasma membrane; IEA:InterPro.
 GO:0005886; C:plasma membrane; IDA:RGD.
 GO:0014069; C:postsynaptic density; IDA:RGD.
 GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
 GO:0008144; F:drug binding; IDA:RGD.
 GO:0016907; F:G-protein coupled acetylcholine receptor activity; IDA:RGD.
 GO:0007274; P:neuromuscular synaptic transmission; IDA:RGD.
 GO:0090316; P:positive regulation of intracellular protein transport; IDA:RGD.
 GO:0043270; P:positive regulation of ion transport; IEA:Compara.
 GO:0060079; P:regulation of excitatory postsynaptic membrane potential; IEA:InterPro.
 GO:0040012; P:regulation of locomotion; IEA:Compara.
 GO:1900274; P:regulation of phospholipase C activity; IEA:InterPro. 
Interpro
 IPR000276; GPCR_Rhodpsn.
 IPR017452; GPCR_Rhodpsn_7TM.
 IPR002228; Musac_Ach_M1_rcpt.
 IPR000995; Musac_Ach_rcpt. 
Pfam
 PF00001; 7tm_1 
SMART
  
PROSITE
 PS00237; G_PROTEIN_RECEP_F1_1
 PS50262; G_PROTEIN_RECEP_F1_2 
PRINTS
 PR00237; GPCRRHODOPSN.
 PR00243; MUSCARINICR.
 PR00538; MUSCRINICM1R.