UniProt Accession

 MED15_HUMAN; Q96RN5; D3DX31; D3DX32; O15413; Q6IC31; Q8NF16; Q96CT0; Q96IH7; Q9P1T3 

Genbank Protein ID

 AF056191; AF328769; AK074268; AK090465; CR456537; CH471176; CH471176; CH471176; CH471176; BC007529; BC013985; U80745 

Genbank Nucleotide ID

 AAC12944.1; AAK58423.1; BAB85034.1; BAC03446.1; CAG30423.1; EAX02951.1; EAX02952.1; EAX02954.1; EAX02956.1; AAH07529.1; AAH13985.1; AAB91443.1 

Protein Name

 Mediator of RNA polymerase II transcription subunit 15 

Protein Synonyms/Alias

 Activator-recruited cofactor 105 kDa component; ARC105; CTG repeat protein 7a; Mediator complex subunit 15; Positive cofactor 2 glutamine/Q-rich-associated protein; PC2 glutamine/Q-rich-associated protein; TPA-inducible gene 1 protein; TIG-1; Trinucleotide repeat-containing gene 7 protein 

Gene Name

 MED15 

Gene Synonyms/Alias

 ARC105; CTG7A; PCQAP; TIG1; TNRC7 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
567	KKDLSKMKSLLDILT	ubiquitination	[1]
578	DILTDPSKRCPLKTL	ubiquitination	[2, 3, 4]

Reference

 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway. 

Sequence Annotation

 REGION 9 73 Interaction with SREBF1.
 MOTIF 547 564 Nuclear localization signal (Potential).
 MOD_RES 603 603 Phosphothreonine.  

Keyword

 3D-structure; Activator; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation; Triplet repeat expansion; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 788 AA 

Protein Sequence

Gene Ontology

 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0016592; C:mediator complex; IEA:InterPro.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; IEA:InterPro.
 GO:0019827; P:stem cell maintenance; IEA:Compara.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome. 

Interpro

 IPR019087; Mediator_Med15_met. 

Pfam

 PF09606; Med15 

SMART

  

PROSITE

  

PRINTS