CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022821
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Coatomer subunit gamma-2 
Protein Synonyms/Alias
 Gamma-2-coat protein; Gamma-2-COP 
Gene Name
 COPG2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
145QAIERYMKQAIVDKVubiquitination[1]
350LAITTLLKTGSESSVubiquitination[2]
414DDGGFEYKRAIVDCIubiquitination[1]
461KILHLLGKEGPRTPVubiquitination[3, 4]
471PRTPVPSKYIRFIFNubiquitination[4, 5]
572YTLEPSEKPFDMKSIubiquitination[3]
599EITLVATKPEKLAPSubiquitination[4, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). 
Sequence Annotation
 REPEAT 64 101 HEAT 1.
 REPEAT 283 320 HEAT 2.
 REPEAT 321 355 HEAT 3.
 REPEAT 356 392 HEAT 4.
 REPEAT 395 430 HEAT 5.
 REPEAT 467 504 HEAT 6.  
Keyword
 Complete proteome; Cytoplasm; Cytoplasmic vesicle; ER-Golgi transport; Golgi apparatus; Membrane; Polymorphism; Protein transport; Reference proteome; Repeat; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 871 AA 
Protein Sequence
MIKKFDKKDE ESGSGSNPFQ HLEKSAVLQE ARIFNETPIN PRRCLHILTK ILYLLNQGEH 60
FGTTEATEAF FAMTRLFQSN DQTLRRMCYL TIKEMATISE DVIIVTSSLT KDMTGKEDVY 120
RGPAIRALCR ITDGTMLQAI ERYMKQAIVD KVSSVSSSAL VSSLHMMKIS YDVVKRWINE 180
AQEAASSDNI MVQYHALGVL YHLRKNDRLA VSKMLNKFTK SGLKSQFAYC MLIRIASRLL 240
KETEDGHESP LFDFIESCLR NKHEMVIYEA ASAIIHLPNC TARELAPAVS VLQLFCSSPK 300
PALRYAAVRT LNKVAMKHPS AVTACNLDLE NLITDSNRSI ATLAITTLLK TGSESSVDRL 360
MKQISSFVSE ISDEFKVVVV QAISALCQKY PRKHSVMMTF LSNMLRDDGG FEYKRAIVDC 420
IISIVEENPE SKEAGLAHLC EFIEDCEHTV LATKILHLLG KEGPRTPVPS KYIRFIFNRV 480
VLENEAVRAA AVSALAKFGA QNESLLPSIL VLLQRCMMDT DDEVRDRATF YLNVLQQRQM 540
ALNATYIFNG LTVSVPGMEK ALHQYTLEPS EKPFDMKSIP LAMAPVFEQK AEITLVATKP 600
EKLAPSRQDI FQEQLAAIPE FLNIGPLFKS SEPVQLTEAE TEYFVRCIKH MFTNHIVFQF 660
DCTNTLNDQL LEKVTVQMEP SDSYEVLSCI PAPSLPYNQP GICYTLVRLP DDDPTAVAGS 720
FSCTMKFTVR DCDPNTGVPD EDGYDDEYVL EDLEVTVSDH IQKVLKPNFA AAWEEVGDTF 780
EKEETFALSS TKTLEEAVNN IITFLGMQPC ERSDKVPENK NSHSLYLAGI FRGGYDLLVR 840
SRLALADGVT MQVTVRSKER TPVDVILASV G 871 
Gene Ontology
 GO:0030126; C:COPI vesicle coat; IDA:UniProtKB.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0006891; P:intra-Golgi vesicle-mediated transport; IDA:UniProtKB.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0006890; P:retrograde vesicle-mediated transport, Golgi to ER; TAS:UniProtKB. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR002553; Clathrin/coatomer_adapt-like_N.
 IPR015873; Clathrin_a/coatomer_app_sub_C.
 IPR009028; Coatomer/calthrin_app_sub_C.
 IPR013041; Coatomer/clathrin_app_Ig-like.
 IPR017106; Coatomer_gsu.
 IPR014863; Coatomer_gsu_app.
 IPR013040; Coatomer_gsu_app_Ig-like-sub. 
Pfam
 PF01602; Adaptin_N
 PF08752; Gamma-COP 
SMART
  
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS