UniProt Accession

 B4DEM7_HUMAN; B4DEM7 

Genbank Protein ID

 AF129075; AK293705 

Genbank Nucleotide ID

 BAG57138.1 

Protein Name

 T-complex protein 1 subunit theta 

Protein Synonyms/Alias

 cDNA FLJ53379, highly similar to T-complex protein 1 subunit theta 

Gene Name

 CCT8 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
18	YRNIQACKELAQTTR	ubiquitination	[1, 2, 3, 4, 5]
35	YGPNGMNKMVINHLE	ubiquitination	[4, 6]
43	MVINHLEKLFVTNDA	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8]
119	GYEIACRKAHEILPN	ubiquitination	[4, 5]
133	NLVCCSAKNLRDIDE	ubiquitination	[2, 4, 5, 6]
152	LRTSIMSKQYGNEVF	ubiquitination	[3, 4, 5, 6]
206	LHGMVFKKETEGDVT	acetylation	[9]
206	LHGMVFKKETEGDVT	ubiquitination	[4, 5]
216	EGDVTSVKDAKIAVY	ubiquitination	[1, 2, 3, 4, 5, 8]
235	DGMITETKGTVLIKT	ubiquitination	[2, 3, 4, 5]
241	TKGTVLIKTAEELMN	ubiquitination	[1, 2, 4, 5, 6, 8]
251	EELMNFSKGEENLMD	acetylation	[9]
251	EELMNFSKGEENLMD	ubiquitination	[1, 2, 3, 4, 5, 8, 10]
277	NVVVTGGKVADMALH	ubiquitination	[2, 4, 5]
288	MALHYANKYNIMLVR	ubiquitination	[4]
299	MLVRLNSKWDLRRLC	acetylation	[5, 9, 11]
299	MLVRLNSKWDLRRLC	ubiquitination	[2, 4, 5]
307	WDLRRLCKTVGATAL	acetylation	[5, 9]
307	WDLRRLCKTVGATAL	ubiquitination	[3, 4, 5, 6, 10]
348	VVVFKHEKEDGAIST	acetylation	[9]
381	DDGVNTFKVLTRDKR	acetylation	[5, 9, 11, 12]
381	DDGVNTFKVLTRDKR	ubiquitination	[1, 2, 3, 4, 5, 6, 8]
402	ATEIELAKQITSYGE	ubiquitination	[2, 4, 5, 6]
420	GLEQYAIKKFAEAFE	ubiquitination	[4]
421	LEQYAIKKFAEAFEA	ubiquitination	[1, 2, 3, 4, 5, 6, 8]
440	LAENSGVKANEVISK	ubiquitination	[1, 3, 4, 5]
447	KANEVISKLYAVHQE	acetylation	[11]
447	KANEVISKLYAVHQE	ubiquitination	[4, 5, 6, 8]
509	VDQIIMAKPAGGPKP	acetylation	[5, 9]
509	VDQIIMAKPAGGPKP	ubiquitination	[2, 4, 5, 6]
515	AKPAGGPKPPSGKKD	ubiquitination	[4, 5, 8]
520	GPKPPSGKKDWDDDQ	ubiquitination	[3, 8]
521	PKPPSGKKDWDDDQN	ubiquitination	[1, 3, 5, 8, 10]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [9] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [10] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [11] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [12] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224] 

Functional Description

  

Sequence Annotation

  

Keyword

 ATP-binding; Chaperone; Complete proteome; Cytoplasm; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 529 AA 

Protein Sequence

Gene Ontology

 GO:0016235; C:aggresome; IDA:HPA.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0045111; C:intermediate filament cytoskeleton; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0006457; P:protein folding; IEA:InterPro. 

Interpro

 IPR012721; Chap_CCT_theta.
 IPR017998; Chaperone_TCP-1.
 IPR002194; Chaperonin_TCP-1_CS.
 IPR002423; Cpn60/TCP-1.
 IPR027409; GroEL-like_apical_dom.
 IPR027413; GROEL-like_equatorial.
 IPR027410; TCP-1-like_intermed. 

Pfam

 PF00118; Cpn60_TCP1 

SMART

  

PROSITE

 PS00750; TCP1_1
 PS00751; TCP1_2
 PS00995; TCP1_3 

PRINTS

 PR00304; TCOMPLEXTCP1.