CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000930
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 PH domain leucine-rich repeat-containing protein phosphatase 1 
Protein Synonyms/Alias
 Pleckstrin homology domain-containing family E member 1; PH domain-containing family E member 1; Suprachiasmatic nucleus circadian oscillatory protein; hSCOP 
Gene Name
 PHLPP1 
Gene Synonyms/Alias
 KIAA0606; PHLPP; PLEKHE1; SCOP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
240RLLQLGHKGGGVVKVubiquitination[1, 2]
246HKGGGVVKVLGQGPGubiquitination[3, 4]
1146PRLVLDHKTLELLNNubiquitination[3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser- 657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and suppression of tumor growth. Controls the phosphorylation of AKT2 and AKT3 more efficiently than that of AKT1. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. May act as a negative regulator of K-Ras signaling in membrane rafts. 
Sequence Annotation
 DOMAIN 536 636 PH.
 REPEAT 638 659 LRR 1.
 REPEAT 661 682 LRR 2.
 REPEAT 692 712 LRR 3.
 REPEAT 715 736 LRR 4.
 REPEAT 738 760 LRR 5.
 REPEAT 761 783 LRR 6.
 REPEAT 784 804 LRR 7.
 REPEAT 808 831 LRR 8.
 REPEAT 832 853 LRR 9.
 REPEAT 873 894 LRR 10.
 REPEAT 895 916 LRR 11.
 REPEAT 918 939 LRR 12.
 REPEAT 941 962 LRR 13.
 REPEAT 963 984 LRR 14.
 REPEAT 987 1008 LRR 15.
 REPEAT 1013 1033 LRR 16.
 REPEAT 1037 1058 LRR 17.
 REPEAT 1061 1082 LRR 18.
 REPEAT 1084 1105 LRR 19.
 REPEAT 1106 1127 LRR 20.
 REPEAT 1129 1150 LRR 21.
 DOMAIN 1165 1420 PP2C-like.
 MOTIF 1715 1717 PDZ-binding.
 METAL 1210 1210 Manganese 1 (By similarity).
 METAL 1210 1210 Manganese 2 (By similarity).
 METAL 1211 1211 Manganese 1; via carbonyl oxygen (By
 METAL 1374 1374 Manganese 2 (By similarity).
 METAL 1413 1413 Manganese 2 (By similarity).  
Keyword
 Apoptosis; Complete proteome; Cytoplasm; Hydrolase; Leucine-rich repeat; Manganese; Membrane; Metal-binding; Nucleus; Polymorphism; Protein phosphatase; Reference proteome; Repeat; Tumor suppressor. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1717 AA 
Protein Sequence
MEPAAAATVQ RLPELGREDR ASAPAAAAAA AAAAAAAAAA LAAAAGGGRS PEPALTPAAP 60
SGGNGSGSGA REEAPGEAPP GPLPGRAGGA GRRRRRGAPQ PIAGGAAPVP GAGGGANSLL 120
LRRGRLKRNL SAAAAAASSS SSSSAAAASH SPGAAGLPAS CSASASLCTR SLDRKTLLLK 180
HRQTLQLQPS DRDWVRHQLQ RGCVHVFDRH MASTYLRPVL CTLDTTAGEV AARLLQLGHK 240
GGGVVKVLGQ GPGAAAAREP AEPPPEAGPR LAPPEPRDSE VPPARSAPGA FGGPPRAPPA 300
DLPLPVGGPG GWSRRASPAP SDSSPGEPFV GGPVSSPRAP RPVVSDTESF SLSPSAESVS 360
DRLDPYSSGG GSSSSSEELE ADAASAPTGV PGQPRRPGHP AQPLPLPQTA SSPQPQQKAP 420
RAIDSPGGAV REGSCEEKAA AAVAPGGLQS TPGRSGVTAE KAPPPPPPPT LYVQLHGETT 480
RRLEAEEKPL QIQNDYLFQL GFGELWRVQE EGMDSEIGCL IRFYAGKPHS TGSSERIQLS 540
GMYNVRKGKM QLPVNRWTRR QVILCGTCLI VSSVKDSLTG KMHVLPLIGG KVEEVKKHQH 600
CLAFSSSGPQ SQTYYICFDT FTEYLRWLRQ VSKVASQRIS SVDLSCCSLE HLPANLFYSQ 660
DLTHLNLKQN FLRQNPSLPA ARGLNELQRF TKLKSLNLSN NHLGDFPLAV CSIPTLAELN 720
VSCNALRSVP AAVGVMHNLQ TFLLDGNFLQ SLPAELENMK QLSYLGLSFN EFTDIPEVLE 780
KLTAVDKLCM SGNCVETLRL QALRKMPHIK HVDLRLNVIR KLIADEVDFL QHVTQLDLRD 840
NKLGDLDAMI FNNIEVLHCE RNQLVTLDIC GYFLKALYAS SNELVQLDVY PVPNYLSYMD 900
VSRNRLENVP EWVCESRKLE VLDIGHNQIC ELPARLFCNS SLRKLLAGHN QLARLPERLE 960
RTSVEVLDVQ HNQLLELPPN LLMKADSLRF LNASANKLES LPPATLSEET NSILQELYLT 1020
NNSLTDKCVP LLTGHPHLKI LHMAYNRLQS FPASKMAKLE ELEEIDLSGN KLKAIPTTIM 1080
NCRRMHTVIA HSNCIEVFPE VMQLPEIKCV DLSCNELSEV TLPENLPPKL QELDLTGNPR 1140
LVLDHKTLEL LNNIRCFKID QPSTGDASGA PAVWSHGYTE ASGVKNKLCV AALSVNNFCD 1200
NREALYGVFD GDRNVEVPYL LQCTMSDILA EELQKTKNEE EYMVNTFIVM QRKLGTAGQK 1260
LGGAAVLCHI KHDPVDPGGS FTLTSANVGK CQTVLCRNGK PLPLSRSYIM SCEEELKRIK 1320
QHKAIITEDG KVNGVTESTR ILGYTFLHPS VVPRPHVQSV LLTPQDEFFI LGSKGLWDSL 1380
SVEEAVEAVR NVPDALAAAK KLCTLAQSYG CHDSISAVVV QLSVTEDSFC CCELSAGGAV 1440
PPPSPGIFPP SVNMVIKDRP SDGLGVPSSS SGMASEISSE LSTSEMSSEV GSTASDEPPP 1500
GALSENSPAY PSEQRCMLHP ICLSNSFQRQ LSSATFSSAF SDNGLDSDDE EPIEGVFTNG 1560
SRVEVEVDIH CSRAKEKEKQ QHLLQVPAEA SDEGIVISAN EDEPGLPRKA DFSAVGTIGR 1620
RRANGSVAPQ ERSHNVIEVA TDAPLRKPGG YFAAPAQPDP DDQFIIPPEL EEEVKEIMKH 1680
HQEQQQQQQP PPPPQLQPQL PRHYQLDQLP DYYDTPL 1717 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0004721; F:phosphoprotein phosphatase activity; IEA:UniProtKB-KW.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0009649; P:entrainment of circadian clock; IEA:Compara.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0008543; P:fibroblast growth factor receptor signaling pathway; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome.
 GO:0008152; P:metabolic process; IEA:GOC.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0048015; P:phosphatidylinositol-mediated signaling; TAS:Reactome. 
Interpro
 IPR001611; Leu-rich_rpt.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR001932; PP2C-like. 
Pfam
 PF00560; LRR_1
 PF00481; PP2C 
SMART
 SM00233; PH
 SM00332; PP2Cc 
PROSITE
 PS51450; LRR
 PS50003; PH_DOMAIN 
PRINTS