CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-035868
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NH(3)-dependent NAD(+) synthetase 
Protein Synonyms/Alias
  
Gene Name
 nadE 
Gene Synonyms/Alias
 EAM_1614 
Created Date
 July 27, 2013 
Organism
 Erwinia amylovora (strain ATCC 49946 / CCPPB 0273 / Ea273 / 27-3) 
NCBI Taxa ID
 716540 
Lysine Modification
Position
Peptide
Type
References
31RTSVEFLKSYLKRYAacetylation[1]
35EFLKSYLKRYAFLKSacetylation[1]
41LKRYAFLKSLVLGVSacetylation[1]
Reference
 [1] Differential lysine acetylation profiles of Erwinia amylovora strains revealed by proteomics.
 Wu X, Vellaichamy A, Wang D, Zamdborg L, Kelleher NL, Huber SC, Zhao Y.
 J Proteomics. 2013 Feb 21;79:60-71. [PMID: 23234799] 
Functional Description
  
Sequence Annotation
 NP_BIND 46 53 ATP (By similarity).
 ACT_SITE 48 48 By similarity.  
Keyword
 ATP-binding; Complete proteome; Ligase; NAD; Nucleotide-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 275 AA 
Protein Sequence
MALQQDIIKA LGVKPTIDVG EEIRTSVEFL KSYLKRYAFL KSLVLGVSGG QDSTLTGKLC 60
QMAVSELRNE TGDDSYQFIA VRLPHGVQAD EHDCQDAIAF IQPDKVITVN IKAAVQASEQ 120
ALREAGMTLS DFIRGNEKAR ERMKAQYSIA GMTAGVVVGT DHAAEAVTGF FTKYGDGGTD 180
INPIFRLNKG QGKQLLKALG CPQHLWLKHP TADLEDDRPG LQDEVALGVT YEMIDRYLQG 240
ENIEPAAAKI IEDWYVKTEH KRRTPVTVFD DFWKG 275 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; IEA:InterPro.
 GO:0008795; F:NAD+ synthase activity; IEA:HAMAP.
 GO:0009435; P:NAD biosynthetic process; IEA:HAMAP. 
Interpro
 IPR022310; NAD/GMP_synthase.
 IPR003694; NAD_synthase.
 IPR022926; NH(3)-dep_NAD(+)_synth.
 IPR014729; Rossmann-like_a/b/a_fold. 
Pfam
 PF02540; NAD_synthase 
SMART
  
PROSITE
  
PRINTS