CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004974
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA-binding protein REB1 
Protein Synonyms/Alias
 QBP 
Gene Name
 REB1 
Gene Synonyms/Alias
 GRF2; YBR049C; YBR0502 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
21SVEEAVLKYVGVGLDacetylation[1]
322KQYSELQKDSMLDSSacetylation[1]
342NYMEVLPKVISQDTQacetylation[1]
477HIFEQRGKWTAEEEQacetylation[1]
807FSSNISMKTEN****sumoylation[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] A proteomic strategy for gaining insights into protein sumoylation in yeast.
 Denison C, Rudner AD, Gerber SA, Bakalarski CE, Moazed D, Gygi SP.
 Mol Cell Proteomics. 2005 Mar;4(3):246-54. [PMID: 15542864] 
Functional Description
 DNA-binding protein that recognizes sites within both the enhancer and the promoter of rRNA transcription, as well as upstream of many genes transcribed by RNA polymerase II. It is essential for cell growth. May stimulate or inhibit transcription. Specifically recognizes the sequence 5'-CCGGGTA-3' or 5'-CGGGTRR- 3' (where R is any purine). A member of the general regulatory factors (GRFs) which act as genome partitioners. Acts as a chromatin insulator which are known as STARs (Subtelomeric anti- silencing region). STARs prevent negative or positive transcription influence by extending across chromotin to a promoter. 
Sequence Annotation
 DOMAIN 470 523 HTH myb-type.
 DOMAIN 692 717 Myb-like.
 DNA_BIND 497 519 H-T-H motif (By similarity).
 MOD_RES 355 355 Phosphoserine.
 CROSSLNK 807 807 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Complete proteome; Direct protein sequencing; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 810 AA 
Protein Sequence
MPSGHNDKNA NQESVEEAVL KYVGVGLDHQ NHDPQLHTKD LENKHSKKQN IVESSSDVDV 60
NNNDDSNRNE DNNDDSENIS ALNANESSSN VDHANSNEQH NAVMDWYLRQ TAHNQQDDED 120
DENNNNTDNG NDSNNHFSQS DIVVDDDDDK NKKDAGVGVD DDHQSMAMAA VAAAYTLSKN 180
NNNNNSIAND SNSRKRQHDN GNNHENSQKK RKNNNDDDDR QIGNVDPELT TLGDADDNDT 240
NNDVIDRDQL VHKAIIDADS ITQHPDFQQY LNTAADTDDN EKLKHIKDHL MRTHGLNHQN 300
KNHNDDTDDL SNSTKQYSEL QKDSMLDSSL NKSRNYMEVL PKVISQDTQP HQQKSPSHDN 360
EAGSVDNSEI SQLLQSAATK ASSLVSLSSS SATPSTSRSN NSKAFDKAED AALERFINEY 420
EAIERLTRQQ VCERIWSSDR PKDNFWNNIY KVLPYRSSSS IYKHMRRKYH IFEQRGKWTA 480
EEEQELAKLC AEKEGQWAEI GKTLGRMPED CRDRWRNYVK CGTNRASNRW SVEEEELLKK 540
VISDMLEEAQ QQQSQLHPNL LEEEQHLLQD DQNDHRNNDE DDDDTASAAA AAAAAIQEQQ 600
QLLQQKQQDD DDAIAAAAAA ASSSLGDNKD EDKPHDSLGI QLDDNSQNSM VPAPSATSTH 660
SKSLSNTIRR HNNKLRKSLM GNGKLDFKDI INWTIVSERM GGTRSRIQCR YKWNKLVKRE 720
AIAKIQTVKD DDMLWIFEKL RDLGITEDSQ VDWDELAALK PGMKLNGLEL KLCYERMKKK 780
VKGYKQKSIN EISKELVDYF SSNISMKTEN 810 
Gene Ontology
 GO:0005654; C:nucleoplasm; IDA:SGD.
 GO:0003682; F:chromatin binding; IEA:InterPro.
 GO:0043565; F:sequence-specific DNA binding; IDA:SGD.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; TAS:SGD.
 GO:0006363; P:termination of RNA polymerase I transcription; TAS:SGD. 
Interpro
 IPR009057; Homeodomain-like.
 IPR017877; Myb-like_dom.
 IPR017930; Myb_dom.
 IPR001005; SANT/Myb. 
Pfam
  
SMART
 SM00717; SANT 
PROSITE
 PS51294; HTH_MYB
 PS50090; MYB_LIKE 
PRINTS