CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010386
UniProt Accession
Genbank Protein ID
 U00089 
Genbank Nucleotide ID
Protein Name
 Ribonucleoside-diphosphate reductase subunit alpha 
Protein Synonyms/Alias
 Ribonucleotide reductase 
Gene Name
 nrdE 
Gene Synonyms/Alias
 MPN_324; MP512 
Created Date
 July 27, 2013 
Organism
 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
NCBI Taxa ID
 272634 
Lysine Modification
Position
Peptide
Type
References
278KRENADEKIRIKSLSacetylation[1]
519ALEKGKFKNFENTKFacetylation[1]
537SYFDKYIKVEPDFWTacetylation[1]
546EPDFWTPKTERVKALacetylation[1]
556RVKALFQKYQVEIPTacetylation[1]
568IPTRENWKELALNIQacetylation[1]
630VPAYQLNKDSYPFYKacetylation[1]
680ATTRDLNKAYIYAFKacetylation[1]
687KAYIYAFKKGCSSIYacetylation[1]
Reference
 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819] 
Functional Description
 Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity). 
Sequence Annotation
 REGION 175 176 Substrate binding (By similarity).
 REGION 384 388 Substrate binding (By similarity).
 REGION 589 593 Substrate binding (By similarity).
 ACT_SITE 384 384 Proton acceptor (By similarity).
 ACT_SITE 386 386 Cysteine radical intermediate (By
 ACT_SITE 388 388 Proton acceptor (By similarity).
 BINDING 159 159 Substrate (By similarity).
 BINDING 204 204 Substrate; via amide nitrogen (By
 DISULFID 176 413 Redox-active (By similarity).  
Keyword
 Allosteric enzyme; ATP-binding; Complete proteome; Disulfide bond; DNA replication; Nucleotide-binding; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 721 AA 
Protein Sequence
MSVKEKIPAF NTQEDLESYI SLNAYTKVYG DFKMDLHAVE AYIQEHVKPK TKVFHSTKER 60
LDFLVKNDYY DENIINMYSF EQFEEITRKA YAYRFRYANF MGAFKFYNAY ALKTFDGKWY 120
LENYEDRVVM NVLFLANGNY NKALKLLKQI ITNRFQPATP TFLNAGRKKR GEFVSCYLLR 180
IEDNMESIGR AITTTLQLSK RDGGVALLLT NIRESGAPIK KIENQSSGII PIMKLLEDSF 240
SYANQLGQRQ GAGAVYLHAH HPDVMQFLDT KRENADEKIR IKSLSLGLVI PDITFTLAKN 300
NEEMALFSPY DVYEEYGKPL SDISVTEMYY ELLANQRIKK TFINARKFFQ TVAELHFESG 360
YPYILFDDTV NRRNAHPNRI VMSNLCSEIV QPSTPSEFHH DLAFKKVGND ISCNLGSLNI 420
AKAMESGPEF SELVKLAIES LDLVSRVSNL ETAPSIQKGN SENHALGLGA MNLHGFLATN 480
QIYYNSPEAI DFTNIFFYTV AYHAFKASSE LALEKGKFKN FENTKFADGS YFDKYIKVEP 540
DFWTPKTERV KALFQKYQVE IPTRENWKEL ALNIQKNGLA NSHLLAIAPT GSISYLSSCT 600
PSLQPVVSPV EVRKEGRLGR IYVPAYQLNK DSYPFYKDGA YELGPEPIIN IAAAAQQHVD 660
QAISLTLFMT DKATTRDLNK AYIYAFKKGC SSIYYVRVRQ EVLEDSEDHT IQMQQCEACV 720
I 721 
Gene Ontology
 GO:0005971; C:ribonucleoside-diphosphate reductase complex; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004748; F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor; IEA:EC.
 GO:0006260; P:DNA replication; IEA:UniProtKB-UniPathway. 
Interpro
 IPR013346; NrdE_NrdA.
 IPR026459; RNR_1b_NrdE.
 IPR000788; RNR_lg_C.
 IPR013509; RNR_lsu_N.
 IPR013554; RNR_N.
 IPR008926; RNR_R1-su_N. 
Pfam
 PF02867; Ribonuc_red_lgC
 PF00317; Ribonuc_red_lgN
 PF08343; RNR_N 
SMART
  
PROSITE
 PS00089; RIBORED_LARGE 
PRINTS
 PR01183; RIBORDTASEM1.