UniProt Accession

 GSH1_MOUSE; P97494; O09166; Q8VCS5 

Genbank Protein ID

 U85414; U85498; BC019374 

Genbank Nucleotide ID

 AAB42020.1; AAB52542.1; AAH19374.1 

Protein Name

 Glutamate--cysteine ligase catalytic subunit 

Protein Synonyms/Alias

 GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase 

Gene Name

 Gclc 

Gene Synonyms/Alias

 Glclc 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
64	SFDHENRKVQLLLNG	ubiquitination	[1]
81	VLETLQEKGERTNPN	ubiquitination	[1]
209	VINVPIFKDKNTPSP	ubiquitination	[1]
211	NVPIFKDKNTPSPFV	ubiquitination	[1]
318	ERGLEPLKNNRFRIS	ubiquitination	[1]
338	SIDSYLSKCGEKYND	ubiquitination	[1]
412	NWQTMRFKPPPPNSD	ubiquitination	[1]
492	YFRKDICKGGNAVVD	acetylation	[2, 3]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

  

Sequence Annotation

 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 5 5 Phosphoserine (By similarity).
 MOD_RES 8 8 Phosphoserine (By similarity).  

Keyword

 Acetylation; ATP-binding; Complete proteome; Glutathione biosynthesis; Ligase; Nucleotide-binding; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 637 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:MGI.
 GO:0017109; C:glutamate-cysteine ligase complex; IDA:MGI.
 GO:0043531; F:ADP binding; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0050662; F:coenzyme binding; ISS:UniProtKB.
 GO:0016595; F:glutamate binding; ISS:UniProtKB.
 GO:0004357; F:glutamate-cysteine ligase activity; ISS:UniProtKB.
 GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
 GO:0045454; P:cell redox homeostasis; ISS:UniProtKB.
 GO:0006534; P:cysteine metabolic process; ISS:UniProtKB.
 GO:0006536; P:glutamate metabolic process; ISS:UniProtKB.
 GO:0006750; P:glutathione biosynthetic process; ISS:UniProtKB.
 GO:0019852; P:L-ascorbic acid metabolic process; IMP:MGI.
 GO:0043066; P:negative regulation of apoptotic process; ISS:UniProtKB.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0031397; P:negative regulation of protein ubiquitination; IMP:MGI.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; ISS:UniProtKB.
 GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; IMP:MGI.
 GO:0050880; P:regulation of blood vessel size; ISS:UniProtKB.
 GO:0051900; P:regulation of mitochondrial depolarization; IGI:MGI.
 GO:0046685; P:response to arsenic-containing substance; IMP:MGI.
 GO:0009408; P:response to heat; ISS:UniProtKB.
 GO:0009725; P:response to hormone stimulus; ISS:UniProtKB.
 GO:0051409; P:response to nitrosative stress; IEA:Compara.
 GO:0006979; P:response to oxidative stress; ISS:UniProtKB.
 GO:0009410; P:response to xenobiotic stimulus; IMP:MGI. 

Interpro

 IPR004308; GCS. 

Pfam

 PF03074; GCS 

SMART

  

PROSITE

  

PRINTS