UniProt Accession

 NAA10_HUMAN; P41227; A6NM98 

Genbank Protein ID

 X77588; U52112; CH471172; BC000308; BC019312 

Genbank Nucleotide ID

 CAA54691.1; EAW72774.1; AAH00308.1; AAH19312.1 

Protein Name

 N-alpha-acetyltransferase 10 

Protein Synonyms/Alias

 N-terminal acetyltransferase complex ARD1 subunit homolog A; NatA catalytic subunit 

Gene Name

 NAA10 

Gene Synonyms/Alias

 ARD1; ARD1A; TE2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
78	HITSLAVKRSHRRLG	ubiquitination	[1, 2, 3, 4, 5]
89	RRLGLAQKLMDQASR	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
105	MIENFNAKYVSLHVR	ubiquitination	[1, 5]
136	QISEVEPKYYADGED	acetylation	[8]
148	GEDAYAMKRDLTQMA	ubiquitination	[1, 2, 3, 4, 5, 6]
179	VLGAIENKVESKGNS	acetylation	[9]
179	VLGAIENKVESKGNS	ubiquitination	[2, 3, 10, 11]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [9] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [10] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [11] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 In complex with NAA15, displays alpha (N-terminal) acetyltransferase activity. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration. 

Sequence Annotation

 DOMAIN 1 152 N-acetyltransferase.
 REGION 1 58 Interaction with NAA15.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 182 182 Phosphoserine.
 MOD_RES 186 186 Phosphoserine.
 MOD_RES 205 205 Phosphoserine.
 MOD_RES 213 213 Phosphoserine.
 MOD_RES 216 216 Phosphoserine.  

Keyword

 Acetylation; Acyltransferase; Alternative splicing; Complete proteome; Cytoplasm; Disease mutation; Nucleus; Phosphoprotein; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 235 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0008080; F:N-acetyltransferase activity; TAS:ProtInc.
 GO:0004596; F:peptide alpha-N-acetyltransferase activity; IEA:EC.
 GO:0006323; P:DNA packaging; TAS:ProtInc.
 GO:0006475; P:internal protein amino acid acetylation; TAS:ProtInc.
 GO:0006474; P:N-terminal protein amino acid acetylation; IDA:UniProtKB. 

Interpro

 IPR016181; Acyl_CoA_acyltransferase.
 IPR000182; GNAT_dom. 

Pfam

 PF00583; Acetyltransf_1 

SMART

  

PROSITE

 PS51186; GNAT 

PRINTS