CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030930
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA topoisomerase 
Protein Synonyms/Alias
  
Gene Name
 TOP3A 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
73IHVCKAVKPNLQVLRubiquitination[1]
162GFVVERFKAIQAFVPubiquitination[1, 2, 3]
226VRSKPKSKWRPQALDubiquitination[1]
239LDTVELEKLASRKLRubiquitination[1]
250RKLRINAKETMRIAEubiquitination[1]
258ETMRIAEKLYTQGYIubiquitination[1]
326HPPIHPTKYTNNLQGubiquitination[1]
451AEHIETIKARMYVGLubiquitination[1]
462YVGLTPDKRFLPGHLubiquitination[1]
507ICDGKKDKFVVLRQQubiquitination[1]
576DMVLKTKKNGGFYLSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; DNA-binding; Isomerase; Reference proteome; Topoisomerase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 906 AA 
Protein Sequence
MNLIIICMAR QSCNPLVLFE AEIEKYCPEN FVDIKKTLER ETRQCQALVI WTDCDREGEN 60
IGFEIIHVCK AVKPNLQVLR ARFSEITPHA VRTACENLTE PDQRVSDAVD VRQELDLRIG 120
AAFTRFQTLR LQRIFPEVLA EQLISYGSCQ FPTLGFVVER FKAIQAFVPE IFHRIKVTHD 180
HKDGIVEFNW KRHRLFNHTA CLVLYQLCVE DPMATVVEVR SKPKSKWRPQ ALDTVELEKL 240
ASRKLRINAK ETMRIAEKLY TQGYISYPRT ETNIFPRDLN LTVLVEQQTP DPRWGAFAQS 300
ILERGGPTPR NGNKSDQAHP PIHPTKYTNN LQGDEQRLYE FIVRHFLACC SQDAQGQETT 360
VEIDIAQERF VAHGLMILAR NYLDVYPYDH WSDKILPVYE QGSHFQPSTV EMVDGETSPP 420
KLLTEADLIA LMEKHGIGTD ATHAEHIETI KARMYVGLTP DKRFLPGHLG MGLVEGYDSM 480
GYEMSKPDLR AELEADLKLI CDGKKDKFVV LRQQVQKYKQ VFIEAVAKAK KLDEALAQYF 540
GNGTELAQQE DIYPAMPEPI RKCPQCNKDM VLKTKKNGGF YLSCMGFPEC RSAVWLPDSV 600
LEASRDSSVC PVCQPHPVYR LKLKFKRGSL PPTMPLEFVC CIGGCDDTLR EILDLRFSGG 660
PPRASQPSGR LQANQSLNRM DNSQHPQPAD SRQTGSSKAL AQTLPPPTAA GESNSVTCNC 720
GQEAVLLTVR KEGPNRGRQF FKCNGGSCNF FLWADSPNPG AGGPPALAYR PLGASLGCPP 780
GPGIHLGGFG NPGDGSGSGT SCLCSQPSVT RTVQKDGPNK GRQFHTCAKP REQQCGFFQW 840
VDENTAPGTS GAPSWTGDRG RTLESEARSK RPRASSSDMG STAKKPRKCS LCHQPGHTRP 900
FCPQNR 906 
Gene Ontology
 GO:0005694; C:chromosome; IEA:InterPro.
 GO:0003917; F:DNA topoisomerase type I activity; IEA:EC.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006265; P:DNA topological change; IEA:InterPro. 
Interpro
 IPR000380; Topo_IA.
 IPR003601; Topo_IA_2.
 IPR023406; Topo_IA_AS.
 IPR013497; Topo_IA_cen.
 IPR013824; Topo_IA_cen_sub1.
 IPR013825; Topo_IA_cen_sub2.
 IPR023405; Topo_IA_core_domain.
 IPR003602; Topo_IA_DNA-bd.
 IPR013498; Topo_IA_Znf.
 IPR006171; Toprim_domain.
 IPR001878; Znf_CCHC.
 IPR010666; Znf_GRF. 
Pfam
 PF01131; Topoisom_bac
 PF01751; Toprim
 PF01396; zf-C4_Topoisom
 PF06839; zf-GRF 
SMART
 SM00437; TOP1Ac
 SM00436; TOP1Bc
 SM00493; TOPRIM
 SM00343; ZnF_C2HC 
PROSITE
 PS00396; TOPOISOMERASE_I_PROK 
PRINTS
 PR00417; PRTPISMRASEI.