CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008946
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Microfibril-associated glycoprotein 3 
Protein Synonyms/Alias
  
Gene Name
 MFAP3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
182SHLRKTEKAINEFFRubiquitination[1, 2]
198EGAEKLQKAFEIAKRubiquitination[1, 2, 3]
204QKAFEIAKRIPIITSubiquitination[2]
213IPIITSAKTLELAKVubiquitination[1, 2]
219AKTLELAKVTQFKTMubiquitination[1, 2, 3, 4, 5]
224LAKVTQFKTMEFARYubiquitination[1, 2]
272DDLGERIKERPALNAubiquitination[2]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Component of the elastin-associated microfibrils. 
Sequence Annotation
 DOMAIN 45 137 Ig-like C2-type.
 CARBOHYD 36 36 N-linked (GlcNAc...) (Potential).
 CARBOHYD 41 41 N-linked (GlcNAc...) (Potential).
 CARBOHYD 110 110 N-linked (GlcNAc...) (Potential).
 DISULFID 73 124 Potential.  
Keyword
 Alternative splicing; Cell membrane; Complete proteome; Disulfide bond; Glycoprotein; Immunoglobulin domain; Membrane; Polymorphism; Reference proteome; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 362 AA 
Protein Sequence
MKLHCCLFTL VASIIVPAAF VLEDVDFDQM VSLEANRSSY NASFPSSFEL SASSHSDDDV 60
IIAKEGTSVS IECLLTASHY EDVHWHNSKG QQLDGRSRGG KWLVSDNFLN ITNVAFDDRG 120
LYTCFVTSPI RASYSVTLRV IFTSGDMSVY YMIVCLIAFT ITLILNVTRL CMMSSHLRKT 180
EKAINEFFRT EGAEKLQKAF EIAKRIPIIT SAKTLELAKV TQFKTMEFAR YIEELARSVP 240
LPPLILNCRA FVEEMFEAVR VDDPDDLGER IKERPALNAQ GGIYVINPEM GRSNSPGGDS 300
DDGSLNEQGQ EIAVQVSVHL QSETKSIDTE SQGSSHFSPP DDIGSAESNC NYKDGAYENC 360
QL 362 
Gene Ontology
 GO:0005576; C:extracellular region; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0030198; P:extracellular matrix organization; TAS:Reactome. 
Interpro
 IPR007110; Ig-like_dom.
 IPR013783; Ig-like_fold.
 IPR013098; Ig_I-set.
 IPR003598; Ig_sub2. 
Pfam
 PF07679; I-set 
SMART
 SM00408; IGc2 
PROSITE
 PS50835; IG_LIKE 
PRINTS