CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018288
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Non-structural maintenance of chromosomes element 1 homolog 
Protein Synonyms/Alias
 Non-SMC element 1 homolog 
Gene Name
 NSMCE1 
Gene Synonyms/Alias
 HSPC333; HSPC337 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
42RLQTHCYKVHDRNATubiquitination[1]
143LKGKKMRKKEAEQVLubiquitination[1]
144KGKKMRKKEAEQVLQubiquitination[1]
152EAEQVLQKFVQNKWLubiquitination[2, 3]
157LQKFVQNKWLIEKEGubiquitination[2, 3]
218MHLPCVAKYFQSNAEubiquitination[1, 2, 3, 4]
241YWPHEIPKVFDPEKEubiquitination[2, 3]
247PKVFDPEKERESGVLubiquitination[1, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT- associated PML bodies (APBs). Has in vitro ubiquitin ligase activity in presence of NDNL2. Is involved in positive regulation of response to DNA damage stimulus. 
Sequence Annotation
 ZN_FING 188 234 NSE1-type.
 REGION 1 102 Interaction with NDNL2.  
Keyword
 3D-structure; Chromosome; Complete proteome; DNA damage; DNA recombination; DNA repair; Ligase; Metal-binding; Nucleus; Polymorphism; Reference proteome; Telomere; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 266 AA 
Protein Sequence
MQGSTRRMGV MTDVHRRFLQ LLMTHGVLEE WDVKRLQTHC YKVHDRNATV DKLEDFINNI 60
NSVLESLYIE IKRGVTEDDG RPIYALVNLA TTSISKMATD FAENELDLFR KALELIIDSE 120
TGFASSTNIL NLVDQLKGKK MRKKEAEQVL QKFVQNKWLI EKEGEFTLHG RAILEMEQYI 180
RETYPDAVKI CNICHSLLIQ GQSCETCGIR MHLPCVAKYF QSNAEPRCPH CNDYWPHEIP 240
KVFDPEKERE SGVLKSNKKS LRSRQH 266 
Gene Ontology
 GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0030915; C:Smc5-Smc6 complex; IDA:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:2001022; P:positive regulation of response to DNA damage stimulus; IMP:UniProtKB. 
Interpro
 IPR011513; Nse1.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR001841; Znf_RING.
 IPR014857; Znf_RING-like. 
Pfam
 PF07574; SMC_Nse1
 PF08746; zf-RING-like 
SMART
  
PROSITE
  
PRINTS