CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008605
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Imitation switch two complex protein 1 
Protein Synonyms/Alias
  
Gene Name
 ITC1 
Gene Synonyms/Alias
 YGL133W; G2842 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
207EEAASSNKSFIVDQGubiquitination[1]
317ASNKKETKKKRKPTEacetylation[2]
319NKKETKKKRKPTEVNacetylation[2]
855VQALQSDKNFLDNCLubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591] 
Functional Description
 Functions as component of the ISW2 complex, which acts in remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. THe ISW2 complex is involved in coordinating transcriptional repression and in inheritance of telomeric silencing. It is involved in repression of MAT a-specific genes, INO1, and early meiotic genes during mitotic growth dependent upon transcription factor UME6 and in a parallel pathway to the RPD3-SIN3 histone deacetylase complex. ITC1 is required for nucleosome-stimulated ATPase activity and chromatin-remodeling activity of the complex. Required for the repression of MATa a-specific genes. 
Sequence Annotation
 DOMAIN 23 130 WAC.
 DOMAIN 423 483 DDT.  
Keyword
 Chromatin regulator; Complete proteome; Nucleus; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1264 AA 
Protein Sequence
MVLYKRKPIL LPDPKPLPLD LNVQVWHIEE TGEWFSSYEE FLERFDFYTR HHFTCEITGT 60
SCLTFFQALD SEETQFKYVE DRFPLKLREP VARFLHFNGI RRLDALVEKV YARFKNDFFP 120
GEVVYLRKQK DSSTTSSNSQ QSTPQPDDMV EINSVGNPGL PQYQYQRRYV IKEKVQFNAT 180
INPESREIVM PAHTKYMLIE EAASSNKSFI VDQGQIYRDR STFTKHLIKC FFKITLQRAS 240
SKMGAPWCVK PEYLAMYGLT MEWPKDMLKY KEDEPVVARR SNSANVSSPE SEKNKRQSKS 300
SGKSNTSNDA SNKKETKKKR KPTEVNDSEN NSSEEDKKKG QNVTSETHSK KRKKEANEEP 360
NTENVESVPT PANAEPQAVT ITSIMDDLAL PYQHPPNIFP NLTYYNEKLE CISLGSTKLS 420
RPFDSFGKLL QAYQFLNTFG SKICLSHFSL DQFITSLKCT DPYELKGEVV LVNIRTQTSK 480
EQEIENNGLP MKNKAETTTE EDSENPSDWQ RNSFIRDMIM KRNSDKVEYK IVHDDPASDD 540
ILDNINHNGS ALLIEVFTAL LRLFINEEGD WSCIVVENWI IDDKGVLMER KDERGEGEAK 600
QKRNAHGYFL QDKEKIDNLK DTLKENATEV QKESDAKNET NSESDSKSDS DSEERDPKLE 660
KCLNYRNVNW IERLTKRQFN NSYWLIILLG VLEDCRHLPM YTEFIDSFIE KIIPKDISAT 720
QLPKQLWRNF CRKLSFSDKV NALWILVDLV SHFSPDIKAA VDDSMELCGQ IRSERFKVAR 780
ELKTEAAVLS NLQGDLQAIQ EKLNKTDENT PSADGADKKD DSESNSEPID LIIIEKKQKL 840
IEEQDKKVQA LQSDKNFLDN CLFENDLQRL KPLGLDRYGN RYFWLDHNGV PFPQYPAGMN 900
ETPKSNNSLS YHSGRLLIQG PKASSAKFFL NVSDEQLSNW QKIRNSEGIS EATREVFGIS 960
KTSSGSYNYV ENGIEVELLD SNDRVNPLIE LTPIQKKIMD ETPSRLLLSP DQWYCIDKLE 1020
DLSRIMDWLD NWGRKEHDLL RQIRPIMERI KSSLSLRDHA LSLTAFTKNE EKLLKELENN 1080
EFTENELNVD SMDVDDKNSG VKSEVDVQVD AEEKREAVID EKLEVIADEL MKLDDSSKTR 1140
NVLNRIQELE DQRDELLEQK KSIINSQRPG ARILARSERK RTKISRGNKV NKQIEILTDL 1200
VNYRHFKAME DVIAWKNVLA NSIWGSSLRK NASGNKRSGV IETVDDKLKD IVGQTSRTVT 1260
PAPN 1264 
Gene Ontology
 GO:0008623; C:CHRAC; IPI:SGD.
 GO:0006338; P:chromatin remodeling; TAS:SGD.
 GO:0006348; P:chromatin silencing at telomere; IMP:SGD.
 GO:0046020; P:negative regulation of transcription from RNA polymerase II promoter by pheromones; IMP:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR004022; DDT_dom.
 IPR018500; DDT_dom_subgr.
 IPR018501; DDT_dom_superfamily.
 IPR013136; WSTF_Acf1_Cbp146. 
Pfam
 PF02791; DDT
 PF10537; WAC_Acf1_DNA_bd 
SMART
 SM00571; DDT 
PROSITE
 PS50827; DDT
 PS51136; WAC 
PRINTS