CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-028319
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 3-isopropylmalate dehydratase large subunit 
Protein Synonyms/Alias
 Alpha-IPM isomerase; Isopropylmalate isomerase 
Gene Name
 leuC 
Gene Synonyms/Alias
 MSMEG_2387; MSMEI_2327 
Created Date
 July 27, 2013 
Organism
 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
NCBI Taxa ID
 246196 
Lysine Modification
Position
Peptide
Type
References
380ADVLRGRKVADGVRMpupylation[1]
Reference
 [1] Proteome-wide identification of mycobacterial pupylation targets.
 Poulsen C, Akhter Y, Jeon AH, Schmitt-Ulms G, Meyer HE, Stefanski A, Stühler K, Wilmanns M, Song YH.
 Mol Syst Biol. 2010 Jul 13;6:386. [PMID: 20631680] 
Functional Description
 Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate (By similarity). 
Sequence Annotation
 METAL 361 361 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 421 421 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 424 424 Iron-sulfur (4Fe-4S) (By similarity).  
Keyword
 4Fe-4S; Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Iron; Iron-sulfur; Leucine biosynthesis; Lyase; Metal-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 484 AA 
Protein Sequence
MDQPTQTSVK PRTLAEKVWD DHVVARGEGE GTAKEPDLIY IDLHLVHEVT SPQAFDGLRL 60
AGRPVRRPDL TIATEDHNVP TIDIDKPIAD PVSRTQVETL RRNCEEFGIR LHPMGDAEQG 120
IVHIIGPQLG LTQPGMTVVC GDSHTSTHGA FGAIAMGIGT SEVEHVMATQ TLSLKPFKTM 180
AVNVDGELPP GVSAKDIILA VIAKIGTGGG QGHVIEYRGS AIRSLSMEGR MTICNMSIEA 240
GARAGMVAPD ETTFEFLKGR PHAPKGADWD AAVAAWSRLR TDEGAEFDTE VYIDASTLSP 300
FVTWGTNPGQ GVPLSEAVPD PELIVDEGAR QAAEKALAYM DLRPGTPMRD IAVDTVFVGS 360
CTNGRIEDLR VVADVLRGRK VADGVRMLIV PGSMRVRAQA ESEGLDQIFT AAGAQWRQAG 420
CSMCLGMNPD QLSPGERCAS TSNRNFEGRQ GKGGRTHLVS PAVAAATAVR GKLASPADLP 480
AAAR 484 
Gene Ontology
 GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:HAMAP.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0009098; P:leucine biosynthetic process; IEA:HAMAP. 
Interpro
 IPR004430; 3-IsopropMal_deHydase_lsu.
 IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
 IPR015937; Acoase/IPM_deHydtase.
 IPR001030; Acoase/IPM_deHydtase_lsu_aba.
 IPR015932; Aconitase/IPMdHydase_lsu_aba_2.
 IPR018136; Aconitase_4Fe-4S_BS. 
Pfam
 PF00330; Aconitase 
SMART
  
PROSITE
 PS00450; ACONITASE_1
 PS01244; ACONITASE_2 
PRINTS
 PR00415; ACONITASE.