CPLM-006032

Genbank Nucleotide ID

14-3-3 protein sigma

Protein Synonyms/Alias

Epithelial cell marker protein 1; Stratifin

Homo sapiens (Human)

Position	Peptide	Type	References
11	ASLIQKAKLAEQAER	ubiquitination	[1, 2, 3, 4]
32	FMKGAVEKGEELSCE	acetylation	[5]
49	NLLSVAYKNVVGGQR	acetylation	[6]
49	NLLSVAYKNVVGGQR	ubiquitination	[1, 2, 3, 7, 8, 9, 10]
68	VLSSIEQKSNEEGSE	acetylation	[5]
68	VLSSIEQKSNEEGSE	ubiquitination	[2, 10]
77	NEEGSEEKGPEVREY	ubiquitination	[2, 10]
122	ESRVFYLKMKGDYYR	ubiquitination	[2, 3, 4, 7, 8]
124	RVFYLKMKGDYYRYL	ubiquitination	[3]
140	EVATGDDKKRIIDSA	acetylation	[5]
159	QEAMDISKKEMPPTN	acetylation	[5]
159	QEAMDISKKEMPPTN	ubiquitination	[10]
160	EAMDISKKEMPPTNP	ubiquitination	[3]

[1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[6] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
[7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[8] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[9] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[10] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]

Functional Description

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity).

MOD_RES 5 5 Phosphoserine.
MOD_RES 64 64 Phosphoserine (By similarity).
MOD_RES 216 216 Phosphoserine (By similarity).
MOD_RES 248 248 Phosphoserine.

3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Secreted.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0030659; C:cytoplasmic vesicle membrane; TAS:Reactome.
GO:0005829; C:cytosol; TAS:Reactome.
GO:0005615; C:extracellular space; TAS:ProtInc.
GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0008426; F:protein kinase C inhibitor activity; TAS:ProtInc.
GO:0016044; P:cellular membrane organization; TAS:Reactome.
GO:0008630; P:intrinsic apoptotic signaling pathway in response to DNA damage; IDA:HGNC.
GO:0030216; P:keratinocyte differentiation; IEA:Compara.
GO:0043616; P:keratinocyte proliferation; IEA:Compara.
GO:0008285; P:negative regulation of cell proliferation; IEA:Compara.
GO:0043154; P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; IDA:HGNC.
GO:0030307; P:positive regulation of cell growth; IEA:Compara.
GO:0046827; P:positive regulation of protein export from nucleus; IEA:Compara.
GO:1900740; P:positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway; TAS:Reactome.
GO:0000079; P:regulation of cyclin-dependent protein serine/threonine kinase activity; IEA:Compara.
GO:0001836; P:release of cytochrome c from mitochondria; IDA:HGNC.
GO:0043588; P:skin development; IEA:Compara.

IPR000308; 14-3-3.
IPR023409; 14-3-3_CS.
IPR023410; 14-3-3_domain.

PS00796; 1433_1
PS00797; 1433_2