UniProt Accession

 DOSC_ECOLI; P0AA89; P77793 

Genbank Protein ID

 U00096; AP009048 

Genbank Nucleotide ID

 AAC74563.3; BAA15155.2 

Protein Name

 Diguanylate cyclase DosC 

Protein Synonyms/Alias

 DGC; Direct oxygen-sensing cyclase 

Gene Name

 dosC 

Gene Synonyms/Alias

 yddV; b1490; JW5241 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
336	VLIIDVDKFKEINDT	acetylation	[1]
338	IIDVDKFKEINDTWG	acetylation	[1]
446	DEALYIAKRRGRNRV	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Globin-coupled heme-based oxygen sensor protein displaying diguanylate cyclase (DGC) activity in response to oxygen availability. Thus, catalyzes the synthesis of cyclic diguanylate (c-di-GMP) via the condensation of 2 GTP molecules. Is involved in the modulation of intracellular c-di-GMP levels, in association with DosP which catalyzes the degradation of c-di-GMP (PDE activity). Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria. DosC regulates biofilm formation through the oxygen-dependent activation of the csgBAC operon, which encodes curli structural subunits, while not affecting the expression of the regulatory operon csgDEFG. DosC, but not the other DGCs in E.coli, also promotes the production of the exopolysaccharide poly-N-acetylglucosamine (PNAG) through up- regulation of the expression of the PNAG biosynthetic pgaABCD operon, independently of CsrA. 

Sequence Annotation

 DOMAIN 325 458 GGDEF.
 ACT_SITE 376 376 Proton acceptor (Potential).
 METAL 98 98 Iron (heme proximal ligand).
 METAL 333 333 Magnesium (By similarity).
 METAL 376 376 Magnesium (By similarity).
 BINDING 341 341 Substrate (By similarity).
 BINDING 350 350 Substrate (By similarity).  

Keyword

 Complete proteome; GTP-binding; Heme; Iron; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome; Transcription; Transcription regulation; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 460 AA 

Protein Sequence

Gene Ontology

 GO:0052621; F:diguanylate cyclase activity; IDA:EcoCyc.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0020037; F:heme binding; IDA:EcoCyc.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0019825; F:oxygen binding; IDA:EcoCyc.
 GO:0016849; F:phosphorus-oxygen lyase activity; IEA:InterPro.
 GO:0009190; P:cyclic nucleotide biosynthetic process; IEA:InterPro.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0015671; P:oxygen transport; IEA:InterPro.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR001054; A/G_cyclase.
 IPR000160; GGDEF_dom.
 IPR009050; Globin-like.
 IPR012292; Globin_dom. 

Pfam

 PF00990; GGDEF 

SMART

 SM00267; GGDEF 

PROSITE

 PS50887; GGDEF 

PRINTS