CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003454
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Siroheme synthase 
Protein Synonyms/Alias
 Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase 
Gene Name
 cysG 
Gene Synonyms/Alias
 b3368; JW3331 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
142LARLLREKLESLLPLacetylation[1]
156LHLGQVAKYAGQLRGacetylation[1]
450RVVGLRDKLNWFSNHacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into siroheme. This reaction consists of the NAD- dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme. 
Sequence Annotation
 REGION 218 457 Uroporphyrinogen-III C-methyltransferase.  
Keyword
 Cobalamin biosynthesis; Complete proteome; Direct protein sequencing; Lyase; Methyltransferase; Multifunctional enzyme; NAD; Oxidoreductase; Porphyrin biosynthesis; Reference proteome; S-adenosyl-L-methionine; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 457 AA 
Protein Sequence
MDHLPIFCQL RDRDCLIVGG GDVAERKARL LLDAGARLTV NALAFIPQFT AWADAGMLTL 60
VEGPFDESLL DTCWLAIAAT DDDALNQRVS EAAEARRIFC NVVDAPKAAS FIMPSIIDRS 120
PLMVAVSSGG TSPVLARLLR EKLESLLPLH LGQVAKYAGQ LRGRVKQQFA TMGERRRFWE 180
KLFVNDRLAQ SLANNDQKAI TETTEQLINE PLDHRGEVVL VGAGPGDAGL LTLKGLQQIQ 240
QADVVVYDRL VSDDIMNLVR RDADRVFVGK RAGYHCVPQE EINQILLREA QKGKRVVRLK 300
GGDPFIFGRG GEELETLCNA GIPFSVVPGI TAASGCSAYS GIPLTHRDYA QSVRLITGHL 360
KTGGELDWEN LAAEKQTLVF YMGLNQAATI QQKLIEHGMP GEMPVAIVEN GTAVTQRVID 420
GTLTQLGELA QQMNSPSLII IGRVVGLRDK LNWFSNH 457 
Gene Ontology
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0043115; F:precorrin-2 dehydrogenase activity; IEA:HAMAP.
 GO:0051266; F:sirohydrochlorin ferrochelatase activity; IEA:EC.
 GO:0004851; F:uroporphyrin-III C-methyltransferase activity; IEA:HAMAP.
 GO:0009236; P:cobalamin biosynthetic process; IEA:HAMAP.
 GO:0019354; P:siroheme biosynthetic process; IEA:HAMAP. 
Interpro
 IPR000878; 4pyrrol_Mease.
 IPR014777; 4pyrrole_Mease_sub1.
 IPR014776; 4pyrrole_Mease_sub2.
 IPR006366; CobA/CysG_C.
 IPR016040; NAD(P)-bd_dom.
 IPR012409; Sirohaem_synth.
 IPR019478; Sirohaem_synthase_dimer_dom.
 IPR006367; Sirohaem_synthase_N.
 IPR003043; Uropor_MeTrfase_CS. 
Pfam
 PF10414; CysG_dimeriser
 PF13241; NAD_binding_7
 PF00590; TP_methylase 
SMART
  
PROSITE
 PS00839; SUMT_1
 PS00840; SUMT_2 
PRINTS