UniProt Accession

 CSTF2_HUMAN; P33240; Q5H951; Q6LA74; Q8N502 

Genbank Protein ID

 M85085; BT009778; Z83819; Z95327; Z95327; Z83819; Z95327; BC017712; BC033135 

Genbank Nucleotide ID

 AAA35724.1; AAP88780.1; CAB06072.2; CAB06072.2; CAI42680.1; CAI42680.1; CAI42681.1; AAH17712.1; AAH33135.1 

Protein Name

 Cleavage stimulation factor subunit 2 

Protein Synonyms/Alias

 CF-1 64 kDa subunit; Cleavage stimulation factor 64 kDa subunit; CSTF 64 kDa subunit; CstF-64 

Gene Name

 CSTF2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
189	VDPEIALKILHRQTN	ubiquitination	[1, 2, 3]
572	ILKEQIQKSTGAP**	ubiquitination	[2]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 

Functional Description

 One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs (By similarity). 

Sequence Annotation

 DOMAIN 16 94 RRM.
 REPEAT 410 414 1; approximate.
 REPEAT 415 419 2.
 REPEAT 420 424 3.
 REPEAT 425 429 4; approximate.
 REPEAT 430 434 5; approximate.
 REPEAT 435 439 6.
 REPEAT 440 444 7.
 REPEAT 445 449 8.
 REPEAT 450 454 9.
 REPEAT 455 459 10; approximate.
 REPEAT 460 464 11.
 REPEAT 465 469 12; approximate.
 REGION 108 248 Interactions with CSTF3 and SYMPK.
 REGION 410 469 12 X 5 AA tandem repeats of M-E-A-R-[AG].
 REGION 514 577 Interaction with RPO2TC1.
 MOD_RES 518 518 Phosphoserine.
 MOD_RES 524 524 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; mRNA processing; Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 577 AA 

Protein Sequence

Gene Ontology

 GO:0071920; C:cleavage body; IDA:UniProtKB.
 GO:0005847; C:mRNA cleavage and polyadenylation specificity factor complex; IDA:UniProtKB.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; TAS:ProtInc.
 GO:0006379; P:mRNA cleavage; TAS:ProtInc.
 GO:0006378; P:mRNA polyadenylation; TAS:ProtInc.
 GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome.
 GO:0006369; P:termination of RNA polymerase II transcription; TAS:Reactome. 

Interpro

 IPR025742; CSTF2_hinge.
 IPR026896; CSTF_C.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 

Pfam

 PF14327; CSTF2_hinge
 PF14304; CSTF_C
 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS