CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011797
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 SUMO-protein ligase SIZ2 
Protein Synonyms/Alias
 SAP and Miz-finger domain-containing protein 2 
Gene Name
 NFI1 
Gene Synonyms/Alias
 SIZ2; YOR156C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
438VNQNHSVKNENQGTVsumoylation[1]
446NENQGTVKQEQDYDSsumoylation[1]
Reference
 [1] Comparative analysis of yeast PIAS-type SUMO ligases in vivo and in vitro.
 Takahashi Y, Toh-E A, Kikuchi Y.
 J Biochem. 2003 Apr;133(4):415-22. [PMID: 12761287] 
Functional Description
 May act as an E3 ligase mediating SUMO/Smt3 attachment to septins. May be involved in chromosome maintenance. 
Sequence Annotation
 DOMAIN 43 77 SAP.
 DOMAIN 139 291 PINIT.
 ZN_FING 323 400 SP-RING-type.  
Keyword
 Complete proteome; Ligase; Metal-binding; Nucleus; Reference proteome; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 726 AA 
Protein Sequence
MASVMSNNNN NNNNNNASYM FTNPLSNTGG GLINEIKDAI NEMEQLKVLE LKQICKSLDL 60
SITGKKAVLQ DRIKQFLRKS CDIGHIDPWR PKAIKILIAK VRINSSLPKY STLWETLKTG 120
AFKHPVASGQ LPVTALQSTA LPPYSQQQAL AYSFTSPFYK PIVQIPDANK KLKQSAGRGC 180
TKMKFKVSKS NHDLLKSNKS YKLYLFSGFS IPFIYETVGH EAIDFPYPCE LVFNGTKLED 240
NVKGLKKQNG TGNPANLTPY LKVPTEMNHL DLHYLNIDKE YSISCFIVEV FSPEALLGKI 300
LKRPKIIKQA TTAYIKRTLN EQDDDDIITT STVLSLQCPI SCTRMKYPAK TDQCKHIQCF 360
DALWFLHSQS QVPTWQCPIC QHPIKFDQLK ISEFVDNIIQ NCNEDVEQVE ISVDGSWKPI 420
HNSSAVITDT VNQNHSVKNE NQGTVKQEQD YDSRNAFDTN LRNGSNHNEP EIISLDSSDD 480
EAFIPASKSF PTHVNPRNDQ LRADIFPSES EGSSDYNPNH TSTPKGSPTM DQDNYQDAFQ 540
MRSFLNQGAT TNINDTPTNN SSINSFVTAT NGDSRIFYNR GPSTPLLPAV LQNLTNQTEA 600
QRNPYGPNYN TTAQDRNLLG IEGDLPPIPP VDPNSEAETE LPTRTTSAAH LPPYIHVSTS 660
GHGDDGKIRK RRHSNVSIYI PKNPYATLMK RRPQANHAIM NKTLAQTNDF NTSAQDNSEV 720
VDLTSD 726 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0000790; C:nuclear chromatin; IDA:SGD.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0019789; F:SUMO ligase activity; IDA:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0030261; P:chromosome condensation; TAS:SGD.
 GO:0007059; P:chromosome segregation; IMP:SGD.
 GO:0016925; P:protein sumoylation; IDA:SGD. 
Interpro
 IPR023321; PINIT.
 IPR003034; SAP_dom.
 IPR027229; SIZ1/SIZ2/Pli1/Gei17.
 IPR004181; Znf_MIZ.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF14324; PINIT
 PF02037; SAP
 PF02891; zf-MIZ 
SMART
 SM00513; SAP 
PROSITE
 PS51466; PINIT
 PS50800; SAP
 PS51044; ZF_SP_RING 
PRINTS