UniProt Accession

 MCFS2_YEAST; P38295; D6VQH2 

Genbank Protein ID

 Z36046; BK006936 

Genbank Nucleotide ID

 CAA85138.1; DAA07292.1 

Protein Name

 Medium-chain fatty acid ethyl ester synthase/esterase 2 

Protein Synonyms/Alias

 Alcohol O-acetyltransferase; Ethanol hexanoyl transferase 1 

Gene Name

 EHT1 

Gene Synonyms/Alias

 YBR177C; YBR1239 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
36	GSIKLHLKDNKEQVD	ubiquitination	[1]
39	KLHLKDNKEQVDFDE	ubiquitination	[1, 2]
85	GAADFSKKFPVFYGR	ubiquitination	[2]
114	WLIDSWKKDYEFDQS	ubiquitination	[1, 2]
127	QSTTSFDKKKFDKDE	acetylation	[3]
297	WSRTLFSKNIAQFLT	ubiquitination	[2]
343	FTPENLIKAKSFKST	ubiquitination	[2]
409	QPYSIVEKNPRILYC	ubiquitination	[2]

Reference

 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Displays enzymatic activity both for medium-chain fatty acid (MCFA) ethyl ester synthesis and hydrolysis (esterase activity). MCFA are toxic for yeast and this enzyme could thus be involved in their detoxification by esterification. 

Sequence Annotation

 ACT_SITE 247 247 Charge relay system (By similarity).
 ACT_SITE 395 395 Charge relay system (By similarity).
 ACT_SITE 423 423 Charge relay system (By similarity).  

Keyword

 Acyltransferase; Complete proteome; Hydrolase; Reference proteome; Serine esterase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 451 AA 

Protein Sequence

Gene Ontology

 GO:0005811; C:lipid particle; IDA:SGD.
 GO:0005741; C:mitochondrial outer membrane; IDA:SGD.
 GO:0004026; F:alcohol O-acetyltransferase activity; IEA:EC.
 GO:0034319; F:alcohol O-butanoyltransferase activity; IDA:SGD.
 GO:0004091; F:carboxylesterase activity; IEA:UniProtKB-KW.
 GO:0017171; F:serine hydrolase activity; ISM:SGD.
 GO:0034338; F:short-chain carboxylesterase activity; IDA:SGD.
 GO:0051792; P:medium-chain fatty acid biosynthetic process; IDA:SGD.
 GO:0051793; P:medium-chain fatty acid catabolic process; IMP:SGD. 

Interpro

 IPR012020; AB-Hydro_YheT.
 IPR000073; AB_hydrolase_1.
 IPR000952; UPF0017_hydro-like_CS. 

Pfam

 PF00561; Abhydrolase_1 

SMART

  

PROSITE

 PS01133; UPF0017 

PRINTS