UniProt Accession

 DAM1_YEAST; P53267; D6VUP6; Q9P422 

Genbank Protein ID

 AF280542; Z72898; BK006941 

Genbank Nucleotide ID

 AAF82130.1; CAA97121.1; DAA08207.1 

Protein Name

 DASH complex subunit DAM1 

Protein Synonyms/Alias

 DUO1 and MPS1-interacting protein 1; Kinetochore assembly protein DAM1; Outer kinetochore protein DAM1 

Gene Name

 DAM1 

Gene Synonyms/Alias

 YGR113W; G6153 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
233	PTNIGMSKSSQGHVT	methylation	[1]

Reference

 [1] The Set1 methyltransferase opposes Ipl1 aurora kinase functions in chromosome segregation.
 Zhang K, Lin W, Latham JA, Riefler GM, Schumacher JM, Chan C, Tatchell K, Hawke DH, Kobayashi R, Dent SY.
 Cell. 2005 Sep 9;122(5):723-34. [PMID: 16143104] 

Functional Description

 Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids. 

Sequence Annotation

 MOD_RES 20 20 Phosphoserine; by IPL1.
 MOD_RES 31 31 Phosphoserine.
 MOD_RES 257 257 Phosphoserine; by IPL1.
 MOD_RES 265 265 Phosphoserine; by IPL1.
 MOD_RES 292 292 Phosphoserine; by IPL1.  

Keyword

 Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis; Nucleus; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 343 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0042729; C:DASH complex; IDA:SGD.
 GO:0005828; C:kinetochore microtubule; IDA:SGD.
 GO:0072686; C:mitotic spindle; IEA:InterPro.
 GO:0051233; C:spindle midzone; IDA:SGD.
 GO:0005200; F:structural constituent of cytoskeleton; IPI:SGD.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0030472; P:mitotic spindle organization in nucleus; IGI:SGD.
 GO:0031110; P:regulation of microtubule polymerization or depolymerization; IDA:SGD. 

Interpro

 IPR013962; DASH_Dam1. 

Pfam

 PF08653; DASH_Dam1 

SMART

  

PROSITE

  

PRINTS