CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010757
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear migration protein NUM1 
Protein Synonyms/Alias
  
Gene Name
 NUM1 
Gene Synonyms/Alias
 PAC12; YDR150W; YD8358.06 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
209LEILTLTKNGMENDLubiquitination[1]
542NKIETPSKQYLSDKAubiquitination[1]
1750VLLRNEEKEALQKKIubiquitination[1]
1867YLKDSLNKMDYVAISubiquitination[1]
2186NIKNSAEKLGYKLVPubiquitination[1]
2196YKLVPFEKSNINLKNubiquitination[1]
2371KNDVDIIKTQAMKYGubiquitination[1]
2466VSHKLNNKTSTVSTKubiquitination[1]
2706TDNMYSGKIFPLPGEubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Controls nuclear migration. NUM1 specifically controls the interaction of the bud neck cytoskeleton with the pre- divisional G2 nucleus. Functions in dynein-anchoring. During late anaphase forms dynein-interacting cortical microtubule capture sites at both cellular poles. This leads to dynein-dependent sliding of the microtubules in the bud. 
Sequence Annotation
 REPEAT 593 656 1.
 REPEAT 657 727 2.
 REPEAT 728 798 3.
 REPEAT 799 862 4.
 REPEAT 863 926 5.
 REPEAT 927 990 6.
 REPEAT 991 1054 7.
 REPEAT 1055 1118 8.
 REPEAT 1119 1182 9.
 REPEAT 1183 1246 10.
 REPEAT 1247 1310 11.
 REPEAT 1311 1374 12.
 REPEAT 1375 1384 13; truncated.
 DOMAIN 2573 2683 PH.
 REGION 593 1384 13 X tandem repeats.
 MOD_RES 611 611 Phosphoserine.
 MOD_RES 675 675 Phosphoserine.
 MOD_RES 746 746 Phosphoserine.
 MOD_RES 881 881 Phosphoserine.
 MOD_RES 945 945 Phosphoserine.
 MOD_RES 1009 1009 Phosphoserine.
 MOD_RES 1201 1201 Phosphoserine.
 MOD_RES 1265 1265 Phosphoserine.
 MOD_RES 1329 1329 Phosphoserine.
 MOD_RES 2162 2162 Phosphoserine.
 MOD_RES 2164 2164 Phosphoserine.
 MOD_RES 2197 2197 Phosphoserine.
 MOD_RES 2217 2217 Phosphoserine.
 MOD_RES 2220 2220 Phosphoserine.
 MOD_RES 2221 2221 Phosphoserine.
 MOD_RES 2360 2360 Phosphoserine.
 MOD_RES 2424 2424 Phosphoserine.
 MOD_RES 2494 2494 Phosphoserine.
 MOD_RES 2545 2545 Phosphoserine.  
Keyword
 Complete proteome; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2748 AA 
Protein Sequence
MSHNNRHKKN NDKDSSAGQY ANSIDNSLSQ ESVSTNGVTR MANLKADECG SGDEGDKTKR 60
FSISSILSKR ETKDVLPEFA GSSSHNGVLT ANSSKDMNFT LELSENLLVE CRKLQSSNEA 120
KNEQIKSLKQ IKESLSDKIE ELTNQKKSFM KELDSTKDLN WDLESKLTNL SMECRQLKEL 180
KKKTEKSWND EKESLKLLKT DLEILTLTKN GMENDLSSQK LHYDKEISEL KERILDLNNE 240
NDRLLISVSD LTSEINSLQS NRTERIKIQK QLDDAKASIS SLKRKVQKKY YQKQHTSDTT 300
VTSDPDSEGT TSEEDIFDIV IEIDHMIETG PSVEDISEDL VKKYSEKNNM ILLSNDSYKN 360
LLQKSESASK PKDDELMTKE VAENLNMIAL PNDDNYSKKE FSLESHIKYL EASGYKVLPL 420
EEFENLNESL SNPSYNYLKE KLQALKKIPI DQSTFNLLKE PTIDFLLPLT SKIDCLIIPT 480
KDYNDLFESV KNPSIEQMKK CLEAKNDLQS NICKWLEERN GCKWLSNDLY FSMVNKIETP 540
SKQYLSDKAK EYDQVLIDTK ALEGLKNPTI DFLREKASAS DYLLLKKEDY VSPSLEYLVE 600
HAKATNHHLL SDSAYEDLVK CKENPDMEFL KEKSAKLGHT VVSNEAYSEL EKKLEQPSLE 660
YLVEHAKATN HHLLSDSAYE DLVKCKENPD MEFLKEKSAK LGHTVVSNEA YSELQRKYSE 720
LEKEVEQPSL AYLVEHAKAT DHHLLSDSAY EDLVKCKENP DVEFLKEKSA KLGHTVVSSE 780
EYSELQRKYS ELEKEVEQPS LAYLVEHAKA TDHHLLSDSA YEELVKCKEN PDMEFLKEKS 840
AKLGHTVVSN EAYSELEKKL EQPSLAYLVE HAKATDHHLL SDSAYEDLVK CKENSDVEFL 900
KEKSAKLGHT VVSNEAYSEL EKKLEQPSLA YLVEHAKATD HHLLSDSAYE DLVKCKENPD 960
MEFLKEKSAK LGHTVVSNEA YSELEKKLEQ PSLEYLVEHA KATNHHLLSD SAYEDLVKCK 1020
ENPDMEFLKE KSAKLGHTVV SNEAYSELEK KLEQPSLEYL VEHAKATNHH LLSDSAYEEL 1080
VKCKENPDVE FLKEKSAKLG HTVVSNEAYS ELEKKLEQPS LEYLVEHAKA TNHHLLSDSA 1140
YEELVKCKEN PDVEFLKEKS AKLGHTVVSN EAYSELEKKL EQPSLAYLVE HAKATDHHLL 1200
SDSAYEDLVK CKENPDVEFL KEKSAKLGHT VVSNEAYSEL EKKLEQPSLA YLVEHAKATD 1260
HHLLSDSAYE DLVKCKENPD MEFLKEKSAK LGHTVVSNEA YSELEKKLEQ PSLEYLVEHA 1320
KATNHHLLSD SAYEDLVKCK ENPDMEFLKE KSAKLGHTVV SNKEYSELEK KLEQPSLEYL 1380
VKHAEQIQSK IISISDFNTL ANPSMEDMAS KLQKLEYQIV SNDEYIALKN TMEKPDVELL 1440
RSKLKGYHII DTTTYNELVS NFNSPTLKFI EEKAKSKGYR LIEPNEYLDL NRIATTPSKE 1500
EIDNFCKQIG CYALDSKEYE RLKNSLENPS KKFIEENAAL LDLVLVDKTE YQAMKDNASN 1560
KKSLIPSTKA LDFVTMPAPQ LASAEKSSLQ KRTLSDIENE LKALGYVAIR KENLPNLEKP 1620
IVDNASKNDV LNLCSKFSLV PLSTEEYDNM RKEHTKILNI LGDPSIDFLK EKCEKYQMLI 1680
ISKHDYEEKQ EAIENPGYEF ILEKASALGY ELVSEVELDR MKQMIDSPDI DYMQEKAARN 1740
EMVLLRNEEK EALQKKIEYP SLTFLIEKAA GMNKILVDQI EYDETIRKCN HPTRMELEES 1800
CHHLNLVLLD QNEYSTLREP LENRNVEDLI NTLSKLNYIA IPNTIYQDLI GKYENPNFDY 1860
LKDSLNKMDY VAISRQDYEL MVAKYEKPQL DYLKISSEKI DHIVVPLSEY NLMVTNYRNP 1920
SLSYLKEKAV LNNHILIKED DYKNILAVSE HPTVIHLSEK ASLLNKVLVD KDDFATMSRS 1980
IEKPTIDFLS TKALSMGKIL VNESTHKRNE KLLSEPDSEF LTMKAKEQGL IIISEKEYSE 2040
LRDQIDRPSL DVLKEKAAIF DSIIVENIEY QQLVNTTSPC PPITYEDLKV YAHQFGMELC 2100
LQKPNKLSGA ERAERIDEQS INTTSSNSTT TSSMFTDALD DNIEELNRVE LQNNEDYTDI 2160
ISKSSTVKDA TIFIPAYENI KNSAEKLGYK LVPFEKSNIN LKNIEAPLFS KDNDDTSVAS 2220
SIDLDHLSRK AEKYGMTLIS DQEFEEYHIL KDNAVNLNGG MEEMNNPLSE NQNLAAKTTN 2280
TAQEGAFQNT VPHNDMDNEE VEYGPDDPTF TVRQLKKPAG DRNLILTSRE KTLLSRDDNI 2340
MSQNEAVYGD DISDSFVDES QEIKNDVDII KTQAMKYGML CIPESNFVGA SYASAQDMSD 2400
IVVLSASYYH NLMSPEDMKW NCVSNEELQA EVKKRGLQIA LTTKEDKKGQ ATASKHEYVS 2460
HKLNNKTSTV STKSGAKKGL AEAAATTAYE DSESHPQIEE QSHRTNHHKH HKRQQSLNSN 2520
STSKTTHSSR NTPASRRDIV ASFMSRAGSA SRTASLQTLA SLNEPSIIPA LTQTVIGEYL 2580
FKYYPRLGPF GFESRHERFF WVHPYTLTLY WSASNPILEN PANTKTKGVA ILGVESVTDP 2640
NPYPTGLYHK SIVVTTETRT IKFTCPTRQR HNIWYNSLRY LLQRNMQGIS LEDIADDPTD 2700
NMYSGKIFPL PGENTKSSSK RLSASRRSVS TRSLRHRVPQ SRSFGNLR 2748 
Gene Ontology
 GO:0005938; C:cell cortex; IDA:SGD.
 GO:0005934; C:cellular bud tip; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0032065; P:cortical protein anchoring; IEA:InterPro.
 GO:0000226; P:microtubule cytoskeleton organization; IMP:SGD.
 GO:0000266; P:mitochondrial fission; IMP:SGD.
 GO:0000001; P:mitochondrion inheritance; IGI:SGD.
 GO:0030473; P:nuclear migration along microtubule; IMP:SGD. 
Interpro
 IPR024774; PH_dom-Mcp5-type.
 IPR001849; Pleckstrin_homology. 
Pfam
 PF12814; Mcp5_PH 
SMART
 SM00233; PH 
PROSITE
 PS50003; PH_DOMAIN 
PRINTS