CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022621
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Junction-mediating and -regulatory protein 
Protein Synonyms/Alias
  
Gene Name
 Jmy 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
493KEICLEQKKHALKEEubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location. 
Sequence Annotation
 DOMAIN 916 933 WH2.
 REGION 1 119 Interaction with p300/EP300.
 REGION 469 558 Interaction with p300/EP300.
 MOD_RES 64 64 Phosphothreonine.
 MOD_RES 75 75 Phosphoserine.
 MOD_RES 969 969 Phosphoserine (By similarity).  
Keyword
 Actin-binding; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; DNA damage; DNA repair; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 983 AA 
Protein Sequence
MSFALEETLE SDWVAVRPHV FDEREKHKFV FIVAWNEIEG KFAITCHNRT AQRQRSGSRE 60
QAGTPASDGS RGPGSPAARG RSEAAASATA ALRSPGPRKS QAWAEGGSPR SARSLKGDPP 120
RGPAGRGPES PLRSPARAKA SPLRRSAESR DAIASATPVP PAPPVPPVSS VRVVSASGAV 180
SEEIEVLEMV REDEAPQPLP DSEQPPSAAE LESSAEECSW AGLFSFQDLR AVHQQLCSVN 240
SQLEPCLPVF PEEPSGMWTV LFGGAPEMTE QEIDALCYQL QVYLGHGLDT CGWKILSQVL 300
FTETDDPEEY YESLSELRQK GYEEVLQRAR RRIQELLDKH KTIESMVELL DLYQMEDEAY 360
SSLAEATTEL YQYLLQPFRD MRELAMLRRQ QIKISMENDY LGPRRIESLQ KEDADWQRKA 420
HMAVLSIQDL TVKYFEITAK AQKAVYDRMR ADQKKFGKAS WAAAAERMEK LQYAVSKETL 480
QMMRAKEICL EQKKHALKEE MQSLQGGTEA IARLDQLESD YYDLQLQLYE VQFEILKCEE 540
LLLTAQLESI KRLISEKRDE VVYYDTYESM EAMLEKEEMA ASVHAQREEL QKLQQKARQL 600
EARRGRVSAK KAYLRNKKEI CIAKHHEKFQ QRFQSEDEYR AHHTIQIKRD KLHDEEERKS 660
AWVSQERQRT LDRLRTFKQR YPGQVILKST RLRVAHSRRK STASPVPCEE QCHSLPTVLQ 720
GQEKTEVGGG GSQLGPSQTA EPQSLVQLED TSSEQLESTS LPPRAVVSSE LPPPQSAPLL 780
TSIDPKPCSV TIDPLPPPLP PTPPPPPPPP PPPPPPLPVA KDNGASTTAE TLEKDALRTE 840
GNERSIPKSA SAPAAHLFDS SQLVSARKKL RKTVEGLQRR RVSSPMDEVL ASLKRGSFHL 900
KKVEQRTLPP FPDEDDSNNI LAQIRKGVKL KKVQKEVLRE SFTLLPDTDP LTRSIHEALR 960
RIKEASPESE DEEEALPCTD WEN 983 
Gene Ontology
 GO:0031252; C:cell leading edge; ISS:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0003713; F:transcription coactivator activity; IDA:MGI.
 GO:0070060; P:'de novo' actin filament nucleation; IDA:UniProtKB.
 GO:0070358; P:actin polymerization-dependent cell motility; IDA:UniProtKB.
 GO:0034314; P:Arp2/3 complex-mediated actin nucleation; IDA:UniProtKB.
 GO:0007050; P:cell cycle arrest; IDA:UniProtKB.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0006917; P:induction of apoptosis; IDA:MGI.
 GO:0051091; P:positive regulation of sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0043620; P:regulation of DNA-dependent transcription in response to stress; TAS:UniProtKB.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IDA:MGI. 
Interpro
 IPR003124; WH2_dom. 
Pfam
  
SMART
 SM00246; WH2 
PROSITE
 PS51082; WH2 
PRINTS