CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012330
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Squalene monooxygenase 
Protein Synonyms/Alias
 Squalene epoxidase; SE 
Gene Name
 SQLE 
Gene Synonyms/Alias
 ERG1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
90SPPESENKEQLEARRubiquitination[1, 2, 3]
148VLSRDGRKVTVIERDubiquitination[1]
157TVIERDLKEPDRIVGubiquitination[1]
268MGVQYKDKETGDIKEubiquitination[1]
290VADGLFSKFRKSLVSubiquitination[1, 4, 5, 6]
318MKNAPQFKANHAELIubiquitination[1]
363LREYMVEKIYPQIPDubiquitination[1]
373PQIPDHLKEPFLEATubiquitination[1]
426GGMTVAFKDIKLWRKubiquitination[1, 4, 6]
433KDIKLWRKLLKGIPDubiquitination[1]
436KLWRKLLKGIPDLYDubiquitination[1, 2, 6]
451DAAIFEAKKSFYWARubiquitination[1]
452AAIFEAKKSFYWARKubiquitination[1]
543KSEPWITKPRALLSSubiquitination[5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway. 
Sequence Annotation
 NP_BIND 126 153 FAD (Potential).  
Keyword
 Complete proteome; Endoplasmic reticulum; FAD; Flavoprotein; Membrane; Microsome; NADP; Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 574 AA 
Protein Sequence
MWTFLGIATF TYFYKKFGDF ITLANREVLL CVLVFLSLGL VLSYRCRHRN GGLLGRQQSG 60
SQFALFSDIL SGLPFIGFFW AKSPPESENK EQLEARRRRK GTNISETSLI GTAACTSTSS 120
QNDPEVIIVG AGVLGSALAA VLSRDGRKVT VIERDLKEPD RIVGEFLQPG GYHVLKDLGL 180
GDTVEGLDAQ VVNGYMIHDQ ESKSEVQIPY PLSENNQVQS GRAFHHGRFI MSLRKAAMAE 240
PNAKFIEGVV LQLLEEDDVV MGVQYKDKET GDIKELHAPL TVVADGLFSK FRKSLVSNKV 300
SVSSHFVGFL MKNAPQFKAN HAELILANPS PVLIYQISSS ETRVLVDIRG EMPRNLREYM 360
VEKIYPQIPD HLKEPFLEAT DNSHLRSMPA SFLPPSSVKK RGVLLLGDAY NMRHPLTGGG 420
MTVAFKDIKL WRKLLKGIPD LYDDAAIFEA KKSFYWARKT SHSFVVNILA QALYELFSAT 480
DDSLHQLRKA CFLYFKLGGE CVAGPVGLLS VLSPNPLVLI GHFFAVAIYA VYFCFKSEPW 540
ITKPRALLSS GAVLYKACSV IFPLIYSEMK YMVH 574 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0004506; F:squalene monooxygenase activity; NAS:UniProtKB.
 GO:0006725; P:cellular aromatic compound metabolic process; IEA:Compara.
 GO:0006695; P:cholesterol biosynthetic process; TAS:Reactome.
 GO:0010033; P:response to organic substance; IEA:Compara. 
Interpro
 IPR001327; Pyr_OxRdtase_NAD-bd_dom.
 IPR003042; Rng_hydrolase-like.
 IPR013698; Squalene_epoxidase. 
Pfam
 PF00070; Pyr_redox
 PF08491; SE 
SMART
  
PROSITE
  
PRINTS
 PR00420; RNGMNOXGNASE.