UniProt Accession

 A8K7Q2_HUMAN; A8K7Q2 

Genbank Protein ID

 AP000926; AK292067 

Genbank Nucleotide ID

 BAF84756.1 

Protein Name

 Heat shock cognate 71 kDa protein 

Protein Synonyms/Alias

 cDNA FLJ77848 

Gene Name

 HSPA8 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
10	NHFIAEFKRKHKKDI	acetylation	[1, 2]
10	NHFIAEFKRKHKKDI	ubiquitination	[3, 4, 5, 6, 7, 8]
12	FIAEFKRKHKKDISE	ubiquitination	[6]
15	EFKRKHKKDISENKR	ubiquitination	[9]
21	KKDISENKRAVRRLR	acetylation	[8]
21	KKDISENKRAVRRLR	ubiquitination	[3, 5, 9, 10]
83	GTLDPVEKALRDAKL	acetylation	[1, 2, 8]
83	GTLDPVEKALRDAKL	ubiquitination	[3, 4, 5, 6, 7, 8, 9]
89	EKALRDAKLDKSQIH	ubiquitination	[5, 6, 7, 8, 9, 11]
92	LRDAKLDKSQIHDIV	acetylation	[8]
92	LRDAKLDKSQIHDIV	ubiquitination	[3, 5, 6, 7, 8, 9]
109	GGSTRIPKIQKLLQD	ubiquitination	[6, 7, 10]
112	TRIPKIQKLLQDFFN	acetylation	[1, 2, 8]
112	TRIPKIQKLLQDFFN	ubiquitination	[3, 5, 6, 7, 8, 9, 10]
121	LQDFFNGKELNKSIN	acetylation	[2, 8]
121	LQDFFNGKELNKSIN	ubiquitination	[3, 5, 6, 7, 9]
125	FNGKELNKSINPDEA	ubiquitination	[3, 4, 6, 7, 9]
187	RNTTIPTKQTQTFTT	ubiquitination	[3, 4, 6, 7, 8, 9, 10]
215	EGERAMTKDNNLLGK	acetylation	[1, 8]
215	EGERAMTKDNNLLGK	ubiquitination	[3, 4, 6, 7, 8, 9, 10]
222	KDNNLLGKFELTGIP	ubiquitination	[3, 7, 8, 9]
261	AVDKSTGKENKITIT	ubiquitination	[6, 7, 8, 10]
264	KSTGKENKITITNDK	ubiquitination	[6, 7, 8, 9, 10]
271	KITITNDKGRLSKED	acetylation	[1, 2, 8]
271	KITITNDKGRLSKED	ubiquitination	[3, 4, 5, 6, 7, 8, 9, 10, 11, 12]
276	NDKGRLSKEDIERMV	acetylation	[1, 2, 8]
276	NDKGRLSKEDIERMV	ubiquitination	[3, 4, 5, 6, 7, 8, 9, 10, 11]
288	RMVQEAEKYKAEDEK	acetylation	[1, 8]
288	RMVQEAEKYKAEDEK	ubiquitination	[3, 4, 5, 6, 7, 8, 9, 10, 11, 12]
290	VQEAEKYKAEDEKQR	ubiquitination	[7, 8, 9, 10]
295	KYKAEDEKQRDKVSS	acetylation	[8]
295	KYKAEDEKQRDKVSS	ubiquitination	[5, 7, 9, 10]
299	EDEKQRDKVSSKNSL	ubiquitination	[5, 10]
303	QRDKVSSKNSLESYA	ubiquitination	[3, 6, 7, 8, 9, 10]
314	ESYAFNMKATVEDEK	ubiquitination	[4, 7, 9]
321	KATVEDEKLQGKIND	acetylation	[13]
321	KATVEDEKLQGKIND	methylation	[14]
321	KATVEDEKLQGKIND	ubiquitination	[6, 7, 8, 9, 10]
325	EDEKLQGKINDEDKQ	acetylation	[13]
325	EDEKLQGKINDEDKQ	methylation	[14]
325	EDEKLQGKINDEDKQ	ubiquitination	[9]
331	GKINDEDKQKILDKC	acetylation	[13]
331	GKINDEDKQKILDKC	methylation	[14]
333	INDEDKQKILDKCNE	acetylation	[13]
333	INDEDKQKILDKCNE	methylation	[14]
337	DKQKILDKCNEIINW	ubiquitination	[6, 8, 9]
347	EIINWLDKNQTAEKE	acetylation	[8]
347	EIINWLDKNQTAEKE	ubiquitination	[3, 5, 6, 7, 8, 9, 10]
353	DKNQTAEKEEFEHQQ	acetylation	[2, 8]
353	DKNQTAEKEEFEHQQ	ubiquitination	[7, 8, 9, 10]
361	EEFEHQQKELEKVCN	acetylation	[1, 8]
361	EEFEHQQKELEKVCN	ubiquitination	[3, 4, 6, 7, 8, 9, 10]
365	HQQKELEKVCNPIIT	acetylation	[1, 2, 8]
365	HQQKELEKVCNPIIT	ubiquitination	[3, 4, 6, 7, 8, 9, 10]
373	VCNPIITKLYQSAGG	ubiquitination	[7, 8, 10]

Reference

 [1] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [9] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [10] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [11] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [12] A data set of human endogenous protein ubiquitination sites.
 Shi Y, Chan DW, Jung SY, Malovannaya A, Wang Y, Qin J.
 Mol Cell Proteomics. 2011 May;10(5):M110.002089. [PMID: 20972266]
 [13] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [14] Mass spectrometry-based identification and characterisation of lysine and arginine methylation in the human proteome.
 Bremang M, Cuomo A, Agresta AM, Stugiewicz M, Spadotto V, Bonaldi T.
 Mol Biosyst. 2013 Jul 30;9(9):2231-47. [PMID: 23748837] 

Functional Description

  

Sequence Annotation

  

Keyword

 ATP-binding; Complete proteome; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 410 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:Compara.
 GO:0070062; C:extracellular vesicular exosome; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0042623; F:ATPase activity, coupled; IEA:Compara.
 GO:0051085; P:chaperone mediated protein folding requiring cofactor; IEA:Compara.
 GO:0051726; P:regulation of cell cycle; IEA:Compara. 

Interpro

 IPR018181; Heat_shock_70_CS.
 IPR013126; Hsp_70_fam. 

Pfam

 PF00012; HSP70 

SMART

  

PROSITE

 PS01036; HSP70_3 

PRINTS

 PR00301; HEATSHOCK70.