CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029063
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RAS guanyl-releasing protein 2 
Protein Synonyms/Alias
 cDNA FLJ35563 fis, clone SPLEN2005342, highly similar to Homo sapiens RAS guanyl releasing protein 2 (calcium and DAG-regulated) (RASGRP2), transcript variant 2, mRNA 
Gene Name
 RASGRP2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
9AGTLDLDKGCTVEELubiquitination[1, 2]
29EAFDDSGKVRDPQLVubiquitination[2]
74NSNSLQVKTCHLVRYubiquitination[2]
128IDSVPTYKWKRQVTQubiquitination[3]
130SVPTYKWKRQVTQRNubiquitination[2]
259HSSISRLKETHSHVSubiquitination[2]
524ALILGIYKQGLKCRAubiquitination[2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
  
Sequence Annotation
  
Keyword
 Calcium; Complete proteome; Guanine-nucleotide releasing factor; Metal-binding; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 610 AA 
Protein Sequence
MAGTLDLDKG CTVEELLRGC IEAFDDSGKV RDPQLVRMFL MMHPWYIPSS QLAAKLLHIY 60
QQSRKDNSNS LQVKTCHLVR YWISAFPAEF DLNPELAEQI KELKALLDQE GNRRHSSLID 120
IDSVPTYKWK RQVTQRNPVG QKKRKMSLLF DHLEPMELAE HLTYLEYRSF CKILFQDYHS 180
FVTHGCTVDN PVLERFISLF NSVSQWVQLM ILSKPTAPQR ALVITHFVHV AEKLLQLQNF 240
NTLMAVVGGL SHSSISRLKE THSHVSPETI KLWEGLTELV TATGNYGNYR RRLAACVGFR 300
FPILGVHLKD LVALQLALPD WLDPARTRLN GAKMKQLFSI LEELAMVTSL RPPVQANPDL 360
LSLLTVSLDQ YQTEDELYQL SLQREPRSKS SPTSPTSCTP PPRPPVLEEW TSAAKPKLDQ 420
ALVVEHIEKM VESVFRNFDV DGDGHISQEE FQIIRGNFPY LSAFGDLDQN QDGCISREEM 480
VSYFLRSSSV LGGRMGFVHN FQESNSLRPV ACRHCKALIL GIYKQGLKCR ACGVNCHKQC 540
KDRLSVECRR RAQSVSLEGS APSPSPMHSH HHRAFSFSLP RPGRRGSRPP AEIREEEVQT 600
VEDGVFDIHL 610 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR000651; Ras-like_Gua-exchang_fac_N.
 IPR008937; Ras_GEF.
 IPR023578; Ras_GEF_dom.
 IPR001895; RasGRF_CDC25. 
Pfam
 PF00130; C1_1
 PF13202; EF_hand_3
 PF00617; RasGEF
 PF00618; RasGEF_N 
SMART
 SM00109; C1
 SM00054; EFh
 SM00147; RasGEF
 SM00229; RasGEFN 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50009; RASGEF_CAT
 PS50212; RASGEF_NTER
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS