CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021606
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transient receptor potential cation channel subfamily V member 4 
Protein Synonyms/Alias
 TrpV4; Osm-9-like TRP channel 4; OTRPC4; Transient receptor potential protein 12; TRP12; Vanilloid receptor-like channel 2; Vanilloid receptor-like protein 2; VRL-2; Vanilloid receptor-related osmotically-activated channel; VR-OAC 
Gene Name
 TRPV4 
Gene Synonyms/Alias
 VRL2; VROAC 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
344ENTKFVTKMYDLLLLubiquitination[1]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8. 
Sequence Annotation
 REPEAT 237 266 ANK 1.
 REPEAT 284 313 ANK 2.
 REPEAT 369 398 ANK 3.
 REGION 812 831 Interaction with calmodulin.
 BINDING 192 192 ATP.
 BINDING 201 201 ATP.
 BINDING 239 239 ATP.
 BINDING 248 248 ATP.
 MOD_RES 824 824 Phosphoserine; by PKC and PKA.  
Keyword
 3D-structure; Alternative splicing; ANK repeat; ATP-binding; Calcium; Calcium channel; Calcium transport; Calmodulin-binding; Cell junction; Cell membrane; Charcot-Marie-Tooth disease; Complete proteome; Disease mutation; Dwarfism; Ion channel; Ion transport; Membrane; Neurodegeneration; Neuropathy; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 871 AA 
Protein Sequence
MADSSEGPRA GPGEVAELPG DESGTPGGEA FPLSSLANLF EGEDGSLSPS PADASRPAGP 60
GDGRPNLRMK FQGAFRKGVP NPIDLLESTL YESSVVPGPK KAPMDSLFDY GTYRHHSSDN 120
KRWRKKIIEK QPQSPKAPAP QPPPILKVFN RPILFDIVSR GSTADLDGLL PFLLTHKKRL 180
TDEEFREPST GKTCLPKALL NLSNGRNDTI PVLLDIAERT GNMREFINSP FRDIYYRGQT 240
ALHIAIERRC KHYVELLVAQ GADVHAQARG RFFQPKDEGG YFYFGELPLS LAACTNQPHI 300
VNYLTENPHK KADMRRQDSR GNTVLHALVA IADNTRENTK FVTKMYDLLL LKCARLFPDS 360
NLEAVLNNDG LSPLMMAAKT GKIGIFQHII RREVTDEDTR HLSRKFKDWA YGPVYSSLYD 420
LSSLDTCGEE ASVLEILVYN SKIENRHEML AVEPINELLR DKWRKFGAVS FYINVVSYLC 480
AMVIFTLTAY YQPLEGTPPY PYRTTVDYLR LAGEVITLFT GVLFFFTNIK DLFMKKCPGV 540
NSLFIDGSFQ LLYFIYSVLV IVSAALYLAG IEAYLAVMVF ALVLGWMNAL YFTRGLKLTG 600
TYSIMIQKIL FKDLFRFLLV YLLFMIGYAS ALVSLLNPCA NMKVCNEDQT NCTVPTYPSC 660
RDSETFSTFL LDLFKLTIGM GDLEMLSSTK YPVVFIILLV TYIILTFVLL LNMLIALMGE 720
TVGQVSKESK HIWKLQWATT ILDIERSFPV FLRKAFRSGE MVTVGKSSDG TPDRRWCFRV 780
DEVNWSHWNQ NLGIINEDPG KNETYQYYGF SHTVGRLRRD RWSSVVPRVV ELNKNSNPDE 840
VVVPLDSMGN PRCDGHQQGY PRKWRTDDAP L 871 
Gene Ontology
 GO:0005929; C:cilium; IEA:Compara.
 GO:0030864; C:cortical actin cytoskeleton; ISS:BHF-UCL.
 GO:0005881; C:cytoplasmic microtubule; IEA:Compara.
 GO:0030175; C:filopodium; ISS:BHF-UCL.
 GO:0005925; C:focal adhesion; ISS:BHF-UCL.
 GO:0030426; C:growth cone; ISS:BHF-UCL.
 GO:0016021; C:integral to membrane; NAS:UniProtKB.
 GO:0030027; C:lamellipodium; ISS:BHF-UCL.
 GO:0032587; C:ruffle membrane; ISS:BHF-UCL.
 GO:0051015; F:actin filament binding; ISS:BHF-UCL.
 GO:0043014; F:alpha-tubulin binding; ISS:BHF-UCL.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0048487; F:beta-tubulin binding; ISS:BHF-UCL.
 GO:0005262; F:calcium channel activity; IDA:UniProtKB.
 GO:0005516; F:calmodulin binding; IMP:UniProtKB.
 GO:0008017; F:microtubule binding; ISS:BHF-UCL.
 GO:0005034; F:osmosensor activity; IEA:Compara.
 GO:0005080; F:protein kinase C binding; ISS:BHF-UCL.
 GO:0042169; F:SH2 domain binding; ISS:BHF-UCL.
 GO:0031532; P:actin cytoskeleton reorganization; ISS:BHF-UCL.
 GO:0007015; P:actin filament organization; ISS:BHF-UCL.
 GO:0070509; P:calcium ion import; ISS:BHF-UCL.
 GO:0008219; P:cell death; IEA:UniProtKB-KW.
 GO:0006884; P:cell volume homeostasis; TAS:UniProtKB.
 GO:0007043; P:cell-cell junction assembly; ISS:UniProtKB.
 GO:0071476; P:cellular hypotonic response; ISS:BHF-UCL.
 GO:0034605; P:cellular response to heat; ISS:UniProtKB.
 GO:0043622; P:cortical microtubule organization; ISS:BHF-UCL.
 GO:0007204; P:elevation of cytosolic calcium ion concentration; IDA:UniProtKB.
 GO:0042538; P:hyperosmotic salinity response; IEA:Compara.
 GO:0046785; P:microtubule polymerization; ISS:BHF-UCL.
 GO:0010977; P:negative regulation of neuron projection development; ISS:BHF-UCL.
 GO:0007231; P:osmosensory signaling pathway; TAS:UniProtKB.
 GO:0031117; P:positive regulation of microtubule depolymerization; ISS:BHF-UCL.
 GO:0047484; P:regulation of response to osmotic stress; IEA:Compara.
 GO:0009612; P:response to mechanical stimulus; TAS:UniProtKB.
 GO:0030103; P:vasopressin secretion; IEA:Compara. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR005821; Ion_trans_dom.
 IPR004729; TRP_channel.
 IPR008347; TRPV1-4_channel.
 IPR008348; TRPV4_channel.
 IPR024862; TRPV_channel. 
Pfam
 PF00023; Ank
 PF00520; Ion_trans 
SMART
 SM00248; ANK 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT 
PRINTS
 PR01768; TRPVRECEPTOR.
 PR01769; VRL2RECEPTOR.