UniProt Accession

 ZNRF1_HUMAN; Q8ND25; D3DUJ9; Q9H083 

Genbank Protein ID

 AF378524; AL136903; AL834440; AC092383; AC099508; CH471114; CH471114; CH471114; BC007235 

Genbank Nucleotide ID

 AAK69753.1; CAB66837.1; CAD39100.1; EAW95668.1; EAW95670.1; EAW95671.1; AAH07235.1 

Protein Name

 E3 ubiquitin-protein ligase ZNRF1 

Protein Synonyms/Alias

 Nerve injury-induced gene 283 protein; Zinc/RING finger protein 1 

Gene Name

 ZNRF1 

Gene Synonyms/Alias

 NIN283 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
144	FSSHSGFKCPICSKS	ubiquitination	[1]
150	FKCPICSKSVASDEM	ubiquitination	[1]
167	HFIMCLSKPRLSYNG	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 E3 ubiquitin-protein ligase that mediates the ubiquitination of AKT1 and GLUL, thereby playing a role in neuron cells differentiation. Plays a role in the establishment and maintenance of neuronal transmission and plasticity. Regulates Schwann cells differentiation by mediating ubiquitination of GLUL. Promotes neurodegeneration by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of AKT1 in axons: degradation of AKT1 prevents AKT1-mediated phosphorylation of GSK3B, leading to GSK3B activation and phosphorylation of DPYSL2/CRMP2 followed by destabilization of microtubule assembly in axons (Probable). 

Sequence Annotation

 ZN_FING 184 225 RING-type; atypical.
 REGION 2 10 Required for endosomal and lysosomal
 MOD_RES 52 52 Phosphoserine.
 MOD_RES 53 53 Phosphoserine.
 MOD_RES 123 123 Phosphoserine (By similarity).
 LIPID 2 2 N-myristoyl glycine (Probable).  

Keyword

 Alternative splicing; Cell junction; Complete proteome; Cytoplasmic vesicle; Endosome; Ligase; Lipoprotein; Lysosome; Membrane; Metal-binding; Myristate; Phosphoprotein; Reference proteome; Synapse; Ubl conjugation pathway; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 227 AA 

Protein Sequence

Gene Ontology

 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0005768; C:endosome; IDA:LIFEdb.
 GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
 GO:0030672; C:synaptic vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0004842; F:ubiquitin-protein ligase activity; ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
 GO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB. 

Interpro

 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD. 

Pfam

 PF13639; zf-RING_2 

SMART

 SM00184; RING 

PROSITE

 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 

PRINTS