UniProt Accession

 RUXF_HUMAN; P62306; A2VCR2; B2R498; Q15356; Q6IBQ1; Q6P4I0 

Genbank Protein ID

 X85372; CR456751; AK311752; CH471054; BC002505; BC063397; BC128452; BC128453 

Genbank Nucleotide ID

 CAA59688.1; CAG33032.1; BAG34695.1; EAW97548.1; AAH02505.3; AAH63397.2; AAI28453.1; AAI28454.1 

Protein Name

 Small nuclear ribonucleoprotein F 

Protein Synonyms/Alias

 snRNP-F; Sm protein F; Sm-F; SmF 

Gene Name

 SNRPF 

Gene Synonyms/Alias

 PBSCF 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
8	MSLPLNPKPFLNGLT	ubiquitination	[1, 2, 3, 4, 5]
17	FLNGLTGKPVMVKLK	ubiquitination	[1, 2, 3, 4, 5]
22	TGKPVMVKLKWGMEY	ubiquitination	[4]
24	KPVMVKLKWGMEYKG	ubiquitination	[1, 2, 3, 4, 5, 6]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5. 

Sequence Annotation

 MOD_RES 2 2 N-acetylserine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; mRNA processing; mRNA splicing; Nucleus; Reference proteome; Ribonucleoprotein; RNA-binding; Spliceosome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 86 AA 

Protein Sequence

Gene Ontology

 GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005689; C:U12-type spliceosomal complex; IDA:UniProtKB.
 GO:0005683; C:U7 snRNP; IDA:UniProtKB.
 GO:0003723; F:RNA binding; IMP:UniProtKB.
 GO:0008334; P:histone mRNA metabolic process; TAS:Reactome.
 GO:0031124; P:mRNA 3'-end processing; TAS:Reactome.
 GO:0034660; P:ncRNA metabolic process; TAS:Reactome.
 GO:0000387; P:spliceosomal snRNP assembly; TAS:Reactome.
 GO:0006369; P:termination of RNA polymerase II transcription; TAS:Reactome. 

Interpro

 IPR010920; LSM_dom.
 IPR001163; Ribonucl_LSM.
 IPR006649; Ribonucl_LSM_euk/arc.
 IPR016487; snRNP_SmF. 

Pfam

 PF01423; LSM 

SMART

 SM00651; Sm 

PROSITE

  

PRINTS