CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011549
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho GTPase-activating protein 1 
Protein Synonyms/Alias
 CDC42 GTPase-activating protein; GTPase-activating protein rhoOGAP; Rho-related small GTPase protein activator; Rho-type GTPase-activating protein 1; p50-RhoGAP 
Gene Name
 ARHGAP1 
Gene Synonyms/Alias
 CDC42GAP; RHOGAP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
48FPKSDDSKSSSPELVubiquitination[1]
59PELVTHLKWDDPYYDubiquitination[2]
80VEVAGDDKYGRKIIVacetylation[3]
80VEVAGDDKYGRKIIVubiquitination[1, 2]
212GIPRQVLKYDDFLKSacetylation[3]
212GIPRQVLKYDDFLKSubiquitination[2, 4, 5]
218LKYDDFLKSTQKSPAubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate. 
Sequence Annotation
 DOMAIN 63 218 CRAL-TRIO.
 DOMAIN 244 431 Rho-GAP.
 MOTIF 228 238 SH3-binding.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 51 51 Phosphoserine.
 MOD_RES 65 65 Phosphotyrosine (By similarity).
 MOD_RES 80 80 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; GTPase activation; Phosphoprotein; Polymorphism; Reference proteome; SH3-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 439 AA 
Protein Sequence
MDPLSELQDD LTLDDTSEAL NQLKLASIDE KNWPSDEMPD FPKSDDSKSS SPELVTHLKW 60
DDPYYDIARH QIVEVAGDDK YGRKIIVFSA CRMPPSHQLD HSKLLGYLKH TLDQYVESDY 120
TLLYLHHGLT SDNKPSLSWL RDAYREFDRK YKKNIKALYI VHPTMFIKTL LILFKPLISF 180
KFGQKIFYVN YLSELSEHVK LEQLGIPRQV LKYDDFLKST QKSPATAPKP MPPRPPLPNQ 240
QFGVSLQHLQ EKNPEQEPIP IVLRETVAYL QAHALTTEGI FRRSANTQVV REVQQKYNMG 300
LPVDFDQYNE LHLPAVILKT FLRELPEPLL TFDLYPHVVG FLNIDESQRV PATLQVLQTL 360
PEENYQVLRF LTAFLVQISA HSDQNKMTNT NLAVVFGPNL LWAKDAAITL KAINPINTFT 420
KFLLDHQGEL FPSPDPSGL 439 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0001726; C:ruffle; IEA:Compara.
 GO:0030675; F:Rac GTPase activator activity; IEA:Compara.
 GO:0005100; F:Rho GTPase activator activity; IDA:MGI.
 GO:0005070; F:SH3/SH2 adaptor activity; TAS:ProtInc.
 GO:0007266; P:Rho protein signal transduction; TAS:ProtInc. 
Interpro
 IPR001251; CRAL-TRIO_dom.
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom. 
Pfam
 PF00620; RhoGAP 
SMART
 SM00324; RhoGAP
 SM00516; SEC14 
PROSITE
 PS50191; CRAL_TRIO
 PS50238; RHOGAP 
PRINTS