CPLM-023781

Genbank Nucleotide ID

Charged multivesicular body protein 3

Protein Synonyms/Alias

Chromatin-modifying protein 3; Neuroendocrine differentiation factor; Vacuolar protein sorting-associated protein 24; hVps24

CGI149; NEDF; VPS24; CGI-149

Homo sapiens (Human)

Position	Peptide	Type	References
179	ITAGALGKAPSKVTD	ubiquitination	[1, 2]
183	ALGKAPSKVTDALPE	ubiquitination	[1]

[1] Quantitative analysis of global ubiquitination in HeLa cells by mass spectrometry.
Meierhofer D, Wang X, Huang L, Kaiser P.
J Proteome Res. 2008 Oct;7(10):4566-76. [PMID: 18781797]
[2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor. Isoform 2 prevents stress- mediated cell death and accumulation of reactive oxygen species when expressed in yeast cells.

REGION 2 113 Intramolecular interaction with C-
REGION 59 64 Important for autoinhibitory function.
REGION 151 222 Interaction with VPS4A.
REGION 151 220 Intramolecular interaction with N-
REGION 168 169 Important for autoinhibitory function.
REGION 203 207 Interaction with STAMBP.
REGION 221 222 Interaction with STAMBP.
MOTIF 201 211 MIT-interacting motif.
BINDING 216 216 STAMBP.
MOD_RES 200 200 Phosphoserine.
LIPID 2 2 N-myristoyl glycine (Potential).
CROSSLNK 179 179 Glycyl lysine isopeptide (Lys-Gly)

3D-structure; Alternative splicing; Apoptosis; Cell cycle; Cell division; Coiled coil; Complete proteome; Cytoplasm; Endosome; Isopeptide bond; Lipoprotein; Membrane; Myristate; Phosphoprotein; Protein transport; Reference proteome; Transport; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0005769; C:early endosome; IEA:Compara.
GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
GO:0030496; C:midbody; IEA:Compara.
GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IEA:Compara.
GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
GO:0016044; P:cellular membrane organization; TAS:Reactome.
GO:0000910; P:cytokinesis; IEA:Compara.
GO:0016197; P:endosomal transport; TAS:Reactome.
GO:0008333; P:endosome to lysosome transport; IEA:Compara.
GO:0015031; P:protein transport; IEA:UniProtKB-KW.
GO:0051036; P:regulation of endosome size; IEA:Compara.